A lectin-like molecule is discharged from mucocysts of Tetrahymena pyriformis in the presence of insulin

Péter Kovács, Werner E G Müller, G. Csaba

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

By use of a monoclonal antibody directed against purified lectin from the sponge Geodia cydonium it was demonstrated that the mucocysts of Tetrahymena pyriformis contain a substance immunologically similar to that found in G. cydonium. In extracts of T. pyriformis the monoclonal antibody recognizes a 36 kDa protein; binding could be abolished by adsorption of the antibody with (i) erode extract, (ii) purified lectin from G. cydonium and (iii) a 29 aa long peptide. In addition the data show that 10-6 M of insulin causes first the release of mucocyst material, which reacts with the lectin antibody, and second its subsequent redistribution on the surface of the somatic cilia and the oral field.

Original languageEnglish
Pages (from-to)487-491
Number of pages5
JournalJournal of Eukaryotic Microbiology
Volume44
Issue number5
Publication statusPublished - Sep 1997

Fingerprint

Cydonia
Rosaceae
Tetrahymena pyriformis
Lectins
lectins
insulin
Monoclonal Antibodies
Insulin
Antibodies
Cilia
monoclonal antibodies
Porifera
Adsorption
antibodies
extracts
cilia
Peptides
mouth
adsorption
peptides

Keywords

  • Evolution
  • Insulin
  • Lectin
  • Mucocyst discharge
  • Receptors
  • Tetrahymena pyriformis

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Applied Microbiology and Biotechnology
  • Agricultural and Biological Sciences (miscellaneous)
  • Microbiology

Cite this

A lectin-like molecule is discharged from mucocysts of Tetrahymena pyriformis in the presence of insulin. / Kovács, Péter; Müller, Werner E G; Csaba, G.

In: Journal of Eukaryotic Microbiology, Vol. 44, No. 5, 09.1997, p. 487-491.

Research output: Contribution to journalArticle

@article{32def0cd33544e489f9542b3932590dd,
title = "A lectin-like molecule is discharged from mucocysts of Tetrahymena pyriformis in the presence of insulin",
abstract = "By use of a monoclonal antibody directed against purified lectin from the sponge Geodia cydonium it was demonstrated that the mucocysts of Tetrahymena pyriformis contain a substance immunologically similar to that found in G. cydonium. In extracts of T. pyriformis the monoclonal antibody recognizes a 36 kDa protein; binding could be abolished by adsorption of the antibody with (i) erode extract, (ii) purified lectin from G. cydonium and (iii) a 29 aa long peptide. In addition the data show that 10-6 M of insulin causes first the release of mucocyst material, which reacts with the lectin antibody, and second its subsequent redistribution on the surface of the somatic cilia and the oral field.",
keywords = "Evolution, Insulin, Lectin, Mucocyst discharge, Receptors, Tetrahymena pyriformis",
author = "P{\'e}ter Kov{\'a}cs and M{\"u}ller, {Werner E G} and G. Csaba",
year = "1997",
month = "9",
language = "English",
volume = "44",
pages = "487--491",
journal = "Journal of Eukaryotic Microbiology",
issn = "1066-5234",
publisher = "Wiley-Blackwell",
number = "5",

}

TY - JOUR

T1 - A lectin-like molecule is discharged from mucocysts of Tetrahymena pyriformis in the presence of insulin

AU - Kovács, Péter

AU - Müller, Werner E G

AU - Csaba, G.

PY - 1997/9

Y1 - 1997/9

N2 - By use of a monoclonal antibody directed against purified lectin from the sponge Geodia cydonium it was demonstrated that the mucocysts of Tetrahymena pyriformis contain a substance immunologically similar to that found in G. cydonium. In extracts of T. pyriformis the monoclonal antibody recognizes a 36 kDa protein; binding could be abolished by adsorption of the antibody with (i) erode extract, (ii) purified lectin from G. cydonium and (iii) a 29 aa long peptide. In addition the data show that 10-6 M of insulin causes first the release of mucocyst material, which reacts with the lectin antibody, and second its subsequent redistribution on the surface of the somatic cilia and the oral field.

AB - By use of a monoclonal antibody directed against purified lectin from the sponge Geodia cydonium it was demonstrated that the mucocysts of Tetrahymena pyriformis contain a substance immunologically similar to that found in G. cydonium. In extracts of T. pyriformis the monoclonal antibody recognizes a 36 kDa protein; binding could be abolished by adsorption of the antibody with (i) erode extract, (ii) purified lectin from G. cydonium and (iii) a 29 aa long peptide. In addition the data show that 10-6 M of insulin causes first the release of mucocyst material, which reacts with the lectin antibody, and second its subsequent redistribution on the surface of the somatic cilia and the oral field.

KW - Evolution

KW - Insulin

KW - Lectin

KW - Mucocyst discharge

KW - Receptors

KW - Tetrahymena pyriformis

UR - http://www.scopus.com/inward/record.url?scp=1842326194&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=1842326194&partnerID=8YFLogxK

M3 - Article

VL - 44

SP - 487

EP - 491

JO - Journal of Eukaryotic Microbiology

JF - Journal of Eukaryotic Microbiology

SN - 1066-5234

IS - 5

ER -