A highly sensitive chemiluminescence immunoassay for the measurement of coagulation factor XIII subunits and their complex in tears

Zsuzsanna Z. Orosz, E. Katona, Andrea Facskó, A. Berta, L. Muszbek

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Blood coagulation factor XIII (FXIII) is a tetramer (A2B2) consisting of catalytic FXIII-A and carrier/protective FXIII-B subunits. Besides stabilizing fibrin and protecting it from fibrinolysis, FXIII is also involved in wound healing and angiogenesis. The latter functions could be important in body fluids other than blood. In this study highly sensitive chemiluminescent ELISAs were developed to measure low concentrations of FXIII-A, FXIII-B and FXIII-A2B2 antigen levels in tiny volumes of tear. For all three assays the limit of quantitation was below 0.02μg/L, within laboratory imprecision did not exceed 12% and the recovery was close to 100%. The calibration curves showed an excellent fitting using second order polynomial equation. In non-stimulated tears from 40 healthy volunteers a low, but consistent amount of FXIII-A and FXIII-B (medians: 1.74μg/L and 5.73μg/L, respectively) was measured, mostly in free non-complexed form. The respective median values normalized for tear protein concentration were 0.23μg FXIII-A/g and 0.85μg FXIII-B/g. There was no gender and age difference and the values showed non-Gaussian distribution. The results prove that FXIII-A and FXIII-B are low concentration components of tear proteome, which in normal conditions might play a role in the repair of corneal micro-injuries. The developed methods provide a tool for monitoring FXIII subunit and complex levels in pathological conditions.

Original languageEnglish
Pages (from-to)87-92
Number of pages6
JournalJournal of Immunological Methods
Volume353
Issue number1-2
DOIs
Publication statusPublished - Feb 2010

Fingerprint

Luminescent Measurements
Factor XIII
Tears
Immunoassay
Body Fluids
Fibrinolysis
Proteome
Fibrin

Keywords

  • Chemiluminescence immunoassay
  • Factor XIII
  • Factor XIII subunits
  • Tears

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

A highly sensitive chemiluminescence immunoassay for the measurement of coagulation factor XIII subunits and their complex in tears. / Orosz, Zsuzsanna Z.; Katona, E.; Facskó, Andrea; Berta, A.; Muszbek, L.

In: Journal of Immunological Methods, Vol. 353, No. 1-2, 02.2010, p. 87-92.

Research output: Contribution to journalArticle

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