A glial fibrillary acidic protein-expressing and tumorigenic cell line derived from an avian sarcoma virus-induced rat astrocytoma

Y. S. Lee, S. H. Bigner, L. F. Eng, P. Molnár, A. Kuruvilla, D. R. Groothuis, D. D. Bigner

Research output: Contribution to journalArticle

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Abstract

A permanent cell line, S635cl5, was derived from an anaplastic astrocytoma induced by the Schmidt-Ruppin strain of avian sarcoma virus (ASV) in a female F-344 rat. Persistent expression of the astrocytic differentiation protein, glial fibrillary acidic protein (GFAP), was detected both in cultured cells after 100 passages in vitro and in transplanted tumors. Subcutaneous and intracerebral transplantation of S635cl5 cells in syngeneic rats resulted in a 100% tumor incidence and a reproducible mortality distribution. S635cl5 cells formed discrete masses after subcutaneous injection but grew intracranially as infiltrative lesions. Tumor blood flow and blood-to-tissue transport studies yield comparable values to other rat glioma models; S635cl 5 intracranial tumors proved to be a homogeneous model with little variation within and between tumors with respect to morphology, GFAP expression, blood flow, and permeability. This cell line provides a GFAP-expressing brain tumor model that extends the use of autochthonous ASV-induced astrocytomas by allowing in vitro and in vivo studies. It may be useful for further studies in neurobiology and brain tumor biology, diagnosis, and therapy.

Original languageEnglish
Pages (from-to)704-720
Number of pages17
JournalJournal of Neuropathology and Experimental Neurology
Volume45
Issue number6
Publication statusPublished - 1986

Fingerprint

Avian Sarcoma Viruses
Glial Fibrillary Acidic Protein
Astrocytoma
Cell Line
Neoplasms
Brain Neoplasms
Neurobiology
Cell Transplantation
Subcutaneous Injections
Glioma
Cultured Cells
Permeability
Mortality
Incidence
Proteins

Keywords

  • Astrocytoma
  • Avian sarcoma virus
  • Fischer-344
  • Glial fibrillary acidic protein
  • Rat

ASJC Scopus subject areas

  • Clinical Neurology
  • Pathology and Forensic Medicine
  • Cellular and Molecular Neuroscience
  • Neurology
  • Neuroscience(all)

Cite this

A glial fibrillary acidic protein-expressing and tumorigenic cell line derived from an avian sarcoma virus-induced rat astrocytoma. / Lee, Y. S.; Bigner, S. H.; Eng, L. F.; Molnár, P.; Kuruvilla, A.; Groothuis, D. R.; Bigner, D. D.

In: Journal of Neuropathology and Experimental Neurology, Vol. 45, No. 6, 1986, p. 704-720.

Research output: Contribution to journalArticle

Lee, Y. S. ; Bigner, S. H. ; Eng, L. F. ; Molnár, P. ; Kuruvilla, A. ; Groothuis, D. R. ; Bigner, D. D. / A glial fibrillary acidic protein-expressing and tumorigenic cell line derived from an avian sarcoma virus-induced rat astrocytoma. In: Journal of Neuropathology and Experimental Neurology. 1986 ; Vol. 45, No. 6. pp. 704-720.
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