A functional study of concanavalin a-histamine binding site overlap in tetrahymena phagocytosis test

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Abstract

1. 1. Treatment with histamine stimulated the phagocytotic activity of the Tetrahymena to a measurable degree, which was still demonstrable after a week (about 40 generations). 2. 2. Concanavalin A, which binds to the same membrane binding site as histamine, inhibited the stimulatory action of subsequently added histamine, but did not in itself influence phagocytotic activity in any way. 3. 3. The inhibitory effect of Con A on the histamine binding site proved to be dose-dependent. 4. 4. These observations stress the importance of investigating the functional context-as sole realistic measure-of receptor-ligand bindings.

Original languageEnglish
Pages (from-to)457-460
Number of pages4
JournalComparative Biochemistry and Physiology -- Part A: Physiology
Volume75
Issue number3
DOIs
Publication statusPublished - 1983

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Tetrahymena
Histamine Receptors
Phagocytosis
Histamine
Concanavalin A
Ligands
Membranes
Binding Sites

ASJC Scopus subject areas

  • Physiology
  • Molecular Biology
  • Biochemistry

Cite this

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abstract = "1. 1. Treatment with histamine stimulated the phagocytotic activity of the Tetrahymena to a measurable degree, which was still demonstrable after a week (about 40 generations). 2. 2. Concanavalin A, which binds to the same membrane binding site as histamine, inhibited the stimulatory action of subsequently added histamine, but did not in itself influence phagocytotic activity in any way. 3. 3. The inhibitory effect of Con A on the histamine binding site proved to be dose-dependent. 4. 4. These observations stress the importance of investigating the functional context-as sole realistic measure-of receptor-ligand bindings.",
author = "G. Csaba and Z. Darvas and V. L{\'a}szl{\'o}",
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T1 - A functional study of concanavalin a-histamine binding site overlap in tetrahymena phagocytosis test

AU - Csaba, G.

AU - Darvas, Z.

AU - László, V.

PY - 1983

Y1 - 1983

N2 - 1. 1. Treatment with histamine stimulated the phagocytotic activity of the Tetrahymena to a measurable degree, which was still demonstrable after a week (about 40 generations). 2. 2. Concanavalin A, which binds to the same membrane binding site as histamine, inhibited the stimulatory action of subsequently added histamine, but did not in itself influence phagocytotic activity in any way. 3. 3. The inhibitory effect of Con A on the histamine binding site proved to be dose-dependent. 4. 4. These observations stress the importance of investigating the functional context-as sole realistic measure-of receptor-ligand bindings.

AB - 1. 1. Treatment with histamine stimulated the phagocytotic activity of the Tetrahymena to a measurable degree, which was still demonstrable after a week (about 40 generations). 2. 2. Concanavalin A, which binds to the same membrane binding site as histamine, inhibited the stimulatory action of subsequently added histamine, but did not in itself influence phagocytotic activity in any way. 3. 3. The inhibitory effect of Con A on the histamine binding site proved to be dose-dependent. 4. 4. These observations stress the importance of investigating the functional context-as sole realistic measure-of receptor-ligand bindings.

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