A follow‐up study of the development of Rauscher erythroleukemia,

Gábor Szabó, László Mátyus

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2 Citations (Scopus)

Abstract

Expression of cell membrane antigens recognized by antiserum raised against purified Friend murine leukemia virus (Fr‐MuLV) envelope glycoprotein (gp71) and by xenotropic MuLV‐coded cell surface antigen (XenCSA) specific antibodies was studied in the course of the development of Rauscher erythroleukemia in the spleen of Balb/c mice. DNA content vs immunofluorescence or light scattering of cells were simultaneously analyzed. At early stages of the disease (4–5 days after infection) the gp71 and XenCSA‐related antigen expression is enhanced mainly on S‐G2/ M‐phase cells as compared to the majority of G1‐phase cells or to the endogenous background of uninfected cells. Later (around 10 days after infection) an approximately tenfold increased gp'71‐related antigen density is reached in every phase of the cell cycle. These data show that the virus‐induced transition from resting to proliferating state is coupled to enhanced expression of both helper and defective viral env‐gene products in the cell membrane of mitotic and G1‐phase cells as well.

Original languageEnglish
Pages (from-to)92-95
Number of pages4
JournalCytometry
Volume5
Issue number1
DOIs
Publication statusPublished - Jan 1984

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Keywords

  • Flow cytometry
  • Rauscher leukemia
  • antigen expression
  • cell cycle
  • gp70

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Biophysics
  • Hematology
  • Endocrinology
  • Cell Biology

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