A follow-up study of the development of Rauscher erythroleukemia.

G. Szabó, L. Mátyus

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Expression of cell membrane antigens recognized by antiserum raised against purified Friend murine leukemia virus (Fr-MuLV) envelope glycoprotein (gp71) and by xenotropic MuLV-coded cell surface antigen (XenCSA) specific antibodies was studied in the course of the development of Rauscher erythroleukemia in the spleen of Balb/c mice. DNA content vs immunofluorescence or light scattering of cells were simultaneously analyzed. At early stages of the disease (4-5 days after infection) the gp71 and XenCSA-related antigen expression is enhanced mainly on S-G2/M-phase cells as compared to the majority of G1-phase cells or to the endogenous background of uninfected cells. Later (around 10 days after infection) an approximately ten-fold increased gp71-related antigen density is reached in every phase of the cell cycle. These data show that the virus-induced transition from resting to proliferating state is coupled to enhanced expression of both helper and defective viral env-gene products in the cell membrane of mitotic and G1-phase cells as well.

Original languageEnglish
Pages (from-to)92-95
Number of pages4
JournalCytometry
Volume5
Issue number1
Publication statusPublished - Jan 1984

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Leukemia, Erythroblastic, Acute
G1 Phase
Surface Antigens
Antigens
Cell Membrane
Friend murine leukemia virus
env Gene Products
G2 Phase
Viral Proteins
Infection
Cell Division
Fluorescent Antibody Technique
Immune Sera
Glycoproteins
Cell Cycle
Spleen
Viruses
Light
Antibodies
DNA

ASJC Scopus subject areas

  • Biophysics
  • Cell Biology
  • Endocrinology
  • Hematology
  • Pathology and Forensic Medicine

Cite this

A follow-up study of the development of Rauscher erythroleukemia. / Szabó, G.; Mátyus, L.

In: Cytometry, Vol. 5, No. 1, 01.1984, p. 92-95.

Research output: Contribution to journalArticle

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