A fluorescence energy transfer system for studying the functional properties of chromatin.

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Abstract

3YI-B-1 rat embryo fibroblasts were used for double fluorescent labeling. The labeling was carried out with fluorescamine and ethidium bromide as an energy donor-acceptor pair. The fluorimetric measurements proved the existence of an energy transfer process. The parameters of this transfer depended on both cell and dye concentrations. It is this character that makes this labeling technique promising as a means of distinction between cells with different structures.

Original languageEnglish
Pages (from-to)25-29
Number of pages5
JournalActa Biochimica et Biophysica Academiae Scientiarum Hungaricae
Volume14
Issue number1-2
Publication statusPublished - 1979

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Energy Transfer
Chromatin
Fluorescamine
Fluorescence
Ethidium
Coloring Agents
Embryonic Structures
Fibroblasts

ASJC Scopus subject areas

  • Medicine(all)

Cite this

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title = "A fluorescence energy transfer system for studying the functional properties of chromatin.",
abstract = "3YI-B-1 rat embryo fibroblasts were used for double fluorescent labeling. The labeling was carried out with fluorescamine and ethidium bromide as an energy donor-acceptor pair. The fluorimetric measurements proved the existence of an energy transfer process. The parameters of this transfer depended on both cell and dye concentrations. It is this character that makes this labeling technique promising as a means of distinction between cells with different structures.",
author = "B. Somogyi and J. Sz{\"o}llősi and I. R{\'e}dai and S. Damjanovich",
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AU - Szöllősi, J.

AU - Rédai, I.

AU - Damjanovich, S.

PY - 1979

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AB - 3YI-B-1 rat embryo fibroblasts were used for double fluorescent labeling. The labeling was carried out with fluorescamine and ethidium bromide as an energy donor-acceptor pair. The fluorimetric measurements proved the existence of an energy transfer process. The parameters of this transfer depended on both cell and dye concentrations. It is this character that makes this labeling technique promising as a means of distinction between cells with different structures.

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