A flow-through enzyme immunoassay for the screening of fumonisins in maize

C. Paepens, S. De Saeger, L. Sibanda, I. Barna-Vetró, I. Léglise, F. Van Hove, C. Van Peteghem

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Abstract

The format of an existing flow-through enzyme immunoassay has been optimised for the detection of fumonisin B1 (FB1) and B2 (FB2) in maize. The visual detection limit i.e. the smallest mycotoxin concentration resulting in no colour development, is 1000 μg/kg. Assay validation was performed using samples spiked with respectively only FB1 and a FB1 + FB2 mixture (ratio 1:1). Results showed that within-day and between-day coefficients of variation ranged from respectively 0.4-10.2% and 1.1-9.0%. Naturally contaminated samples were screened with the developed flow-through method and results were compared with fumonisin contamination values obtained by a validated HPLC method. The assay was demonstrated to be accurate and reliable giving no false compliant and only a low percentage of false non-compliant results. The described method offers a simple, rapid and cost-effective screening tool, thus contributing to a better consumers' health protection.

Original languageEnglish
Pages (from-to)229-235
Number of pages7
JournalAnalytica Chimica Acta
Volume523
Issue number2
DOIs
Publication statusPublished - Oct 11 2004

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Keywords

  • Fumonisins
  • HPLC
  • Rapid screening method

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy

Cite this

Paepens, C., Saeger, S. D., Sibanda, L., Barna-Vetró, I., Léglise, I., Van Hove, F., & Peteghem, C. V. (2004). A flow-through enzyme immunoassay for the screening of fumonisins in maize. Analytica Chimica Acta, 523(2), 229-235. https://doi.org/10.1016/j.aca.2004.07.044