A FISH karyotype to study chromosome polymorphisms for the Elytrigia elongata E genome

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20 Citations (Scopus)

Abstract

Elytrigia elongata (Host) Nevski(= Agropyron elongatum, Thinopyrum elongatum, 2n = 2x = 14, EE) has long been used as a source of various types of resistance for wheat improvement, and numerous transfers have been made. However, despite heavy use, no high-resolution karyotype exists. We characterized the E. elongata karyotype of several accessions applying highly repetitive DNA sequences as mcFISH probes for chromosome identification. The complete E. elongata disomic chromosome addition series and 11 ditelosomic addition lines in Chinese Spring wheat were exposed to sequential GISH-mcFISH. Based on the mcFISH results, each complete chromosome and each telocentric studied was unambiguously identified. The validation of the karyotype in 4 E. elongata accessions with different geographical origins showed extensive variations in the probe hybridization patterns, but this did not prevent chromosome identification. The established karyotype will be useful for the rapid identification of potential donor chromosomes in wheat improvement programs, allowing appropriate alien transfer.

Original languageEnglish
Pages (from-to)138-144
Number of pages7
JournalCytogenetic and Genome Research
Volume136
Issue number2
DOIs
Publication statusPublished - Mar 2012

Keywords

  • 18S-5.8S-26S rDNA
  • Afa family
  • Chromosome polymorphism
  • Elytrigia elongata
  • mcFISH karyotype
  • pSc119.2

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Genetics(clinical)

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