A DNA element responsible for the different tissue specificities of Friend and Moloney retroviral enhancers

H. J. Thiesen, Z. Bösze, L. Henry, P. Charnay

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The transcriptional enhancers of the Moloney murine sarcoma virus (MuSV) and Moloney murine leukemia virus (MuLV) have different cell type specificities from that of the Friend MuLV. While the three enhancers are approximately equally active in erythroid cells, the Moloney MuSV and Moloney MuLV enhancers are 20- to 40-fold more active than the Friend MuLV enhancer in T-lymphoid cells. Using mutant enhancers, we have shown that specific differences between the nucleotide sequences of the Moloney MuSV and Friend MuLV enhancers are responsible for their different activities in T cells. Our data allow the localization of a DNA element, repeated several times within the enhancer, which modulates the activity of the enhancer in T cells without affecting it in erythroid cells. This element therefore appears to be one of the determinants of the tissue specificity of the enhancer.

Original languageEnglish
Pages (from-to)614-618
Number of pages5
JournalJournal of Virology
Volume62
Issue number2
Publication statusPublished - 1988

Fingerprint

Moloney murine sarcoma virus
Murine leukemia virus
Friend murine leukemia virus
Organ Specificity
Moloney murine leukemia virus
Erythroid Cells
DNA
T-Lymphocytes
T-lymphocytes
cells
Lymphocytes
tissues
nucleotide sequences
mutants

ASJC Scopus subject areas

  • Immunology

Cite this

A DNA element responsible for the different tissue specificities of Friend and Moloney retroviral enhancers. / Thiesen, H. J.; Bösze, Z.; Henry, L.; Charnay, P.

In: Journal of Virology, Vol. 62, No. 2, 1988, p. 614-618.

Research output: Contribution to journalArticle

@article{cc9e4decd7f74e16b7a4848027e21f4f,
title = "A DNA element responsible for the different tissue specificities of Friend and Moloney retroviral enhancers",
abstract = "The transcriptional enhancers of the Moloney murine sarcoma virus (MuSV) and Moloney murine leukemia virus (MuLV) have different cell type specificities from that of the Friend MuLV. While the three enhancers are approximately equally active in erythroid cells, the Moloney MuSV and Moloney MuLV enhancers are 20- to 40-fold more active than the Friend MuLV enhancer in T-lymphoid cells. Using mutant enhancers, we have shown that specific differences between the nucleotide sequences of the Moloney MuSV and Friend MuLV enhancers are responsible for their different activities in T cells. Our data allow the localization of a DNA element, repeated several times within the enhancer, which modulates the activity of the enhancer in T cells without affecting it in erythroid cells. This element therefore appears to be one of the determinants of the tissue specificity of the enhancer.",
author = "Thiesen, {H. J.} and Z. B{\"o}sze and L. Henry and P. Charnay",
year = "1988",
language = "English",
volume = "62",
pages = "614--618",
journal = "Journal of Virology",
issn = "0022-538X",
publisher = "American Society for Microbiology",
number = "2",

}

TY - JOUR

T1 - A DNA element responsible for the different tissue specificities of Friend and Moloney retroviral enhancers

AU - Thiesen, H. J.

AU - Bösze, Z.

AU - Henry, L.

AU - Charnay, P.

PY - 1988

Y1 - 1988

N2 - The transcriptional enhancers of the Moloney murine sarcoma virus (MuSV) and Moloney murine leukemia virus (MuLV) have different cell type specificities from that of the Friend MuLV. While the three enhancers are approximately equally active in erythroid cells, the Moloney MuSV and Moloney MuLV enhancers are 20- to 40-fold more active than the Friend MuLV enhancer in T-lymphoid cells. Using mutant enhancers, we have shown that specific differences between the nucleotide sequences of the Moloney MuSV and Friend MuLV enhancers are responsible for their different activities in T cells. Our data allow the localization of a DNA element, repeated several times within the enhancer, which modulates the activity of the enhancer in T cells without affecting it in erythroid cells. This element therefore appears to be one of the determinants of the tissue specificity of the enhancer.

AB - The transcriptional enhancers of the Moloney murine sarcoma virus (MuSV) and Moloney murine leukemia virus (MuLV) have different cell type specificities from that of the Friend MuLV. While the three enhancers are approximately equally active in erythroid cells, the Moloney MuSV and Moloney MuLV enhancers are 20- to 40-fold more active than the Friend MuLV enhancer in T-lymphoid cells. Using mutant enhancers, we have shown that specific differences between the nucleotide sequences of the Moloney MuSV and Friend MuLV enhancers are responsible for their different activities in T cells. Our data allow the localization of a DNA element, repeated several times within the enhancer, which modulates the activity of the enhancer in T cells without affecting it in erythroid cells. This element therefore appears to be one of the determinants of the tissue specificity of the enhancer.

UR - http://www.scopus.com/inward/record.url?scp=0023838970&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023838970&partnerID=8YFLogxK

M3 - Article

C2 - 2826819

AN - SCOPUS:0023838970

VL - 62

SP - 614

EP - 618

JO - Journal of Virology

JF - Journal of Virology

SN - 0022-538X

IS - 2

ER -