A detailed procedure and dissection guide for the isolation of spiral ganglion cells of the guinea pig for electrophysiological experiments

Zsolt Szabó, Csaba Harasztosi, Géza Szûcs, István Sziklai, Zoltán Rusznák

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

In the present study step-by-step instructions are provided for a preparative technique employed for the removal of the spiral ganglion from the inner ear of the guinea pig. Removal of the temporal bone is followed by opening of the bulla and excision of the modiolus. All major steps of the technique are illustrated with photographs. A procedure to obtain surviving, acutely separated spiral ganglion neurones is also described. By this procedure small tissue pieces are removed from the modiolus which contain the spiral ganglion neurones. The tissue fragments then undergo a mild enzyme treatment (collagenase and pronase). After the enzyme exposure, the tissue pieces are gently triturated, and the isolated cells are allowed to settle. Poly-D-lysine ensured the firm attachment of the spiral ganglion cells to the cover-slips. The application of this adhesive coating seemed to be desirable in functional studies when microelectrode techniques and/or rapid exchange of the extracellular solution were employed.

Original languageEnglish
Pages (from-to)139-147
Number of pages9
JournalBrain Research Protocols
Volume10
Issue number3
DOIs
Publication statusPublished - Feb 1 2003

Keywords

  • Cell isolation
  • Spiral ganglion

ASJC Scopus subject areas

  • Neuroscience(all)

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