A detailed mechanism of the oxidative half-reaction of d-amino acid oxidase

Another route for flavin oxidation

Dóra Judit Kiss, G. Ferenczy

Research output: Contribution to journalArticle

Abstract

D-Amino acid oxidase (DAAO) is a flavoenzyme whose inhibition is expected to have therapeutic potential in schizophrenia. DAAO catalyses hydride transfer from the substrate to the flavin in the reductive half-reaction, and the flavin is reoxidized by O2 in the oxidative half-reaction. Quantum mechanical/molecular mechanical calculations were performed and their results together with available experimental information were used to elucidate the detailed mechanism of the oxidative half-reaction. The reaction starts with a single electron transfer from FAD to O2, followed by triplet-singlet transition. FAD oxidation is completed by a proton coupled electron transfer to the oxygen species and the reaction terminates with H2O2 formation by proton transfer from the oxidized substrate to the oxygen species via a chain of water molecules. The substrate plays a double role by facilitating the first electron transfer and by providing a proton in the last step. The mechanism differs from the oxidative half-reaction of other oxidases.

Original languageEnglish
Pages (from-to)7973-7984
Number of pages12
JournalOrganic and Biomolecular Chemistry
Volume17
Issue number34
DOIs
Publication statusPublished - Jan 1 2019

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oxidase
D-Amino-Acid Oxidase
amino acids
Oxidoreductases
Flavin-Adenine Dinucleotide
routes
Amino Acids
Oxidation
oxidation
Electrons
Protons
Substrates
Oxygen
electron transfer
Proton transfer
Hydrides
protons
schizophrenia
Molecules
Water

ASJC Scopus subject areas

  • Biochemistry
  • Physical and Theoretical Chemistry
  • Organic Chemistry

Cite this

A detailed mechanism of the oxidative half-reaction of d-amino acid oxidase : Another route for flavin oxidation. / Kiss, Dóra Judit; Ferenczy, G.

In: Organic and Biomolecular Chemistry, Vol. 17, No. 34, 01.01.2019, p. 7973-7984.

Research output: Contribution to journalArticle

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