A deficiency of manganese ions in the presence of high sugar concentrations is the critical parameter for achieving high yields of itaconic acid by Aspergillus terreus

Levente Karaffa, Rafael Díaz, Benedek Papp, Erzsébet Fekete, Erzsébet Sándor, Christian P. Kubicek

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Itaconic acid (IA), an unsaturated dicarboxylic acid with a high potential as a platform for chemicals derived from sugars, is industrially produced by large-scale submerged fermentation by Aspergillus terreus. Although the biochemical pathway and the physiology leading to IA is almost the same as that leading to citric acid production in Aspergillus niger, published data for the volumetric (g L−1) and the specific yield (mol/mol carbon source) of IA are significantly lower than for citric acid. Citric acid is known to accumulate to high levels only when a number of nutritional parameters are carefully adjusted, of which the concentration of the carbon source and that of manganese ions in the medium are particularly important. We have therefore investigated whether a variation in these two parameters may enhance IA production and yield by A. terreus. We show that manganese ion concentrations < 3 ppb are necessary to obtain highest yields. Highest yields were also dependent on the concentration of the carbon source (d-glucose), and highest yields (0.9) were only obtained at concentrations of 12–20 % (w/v), thus allowing the accumulation of up to 130 g L−1 IA. These findings perfectly mirror those obtained when these parameters are varied in citric acid production by A. niger, thus showing that the physiology of both processes is widely identical. Consequently, applying the fermentation technology established for citric acid production by A. niger citric acid production to A. terreus should lead to high yields of IA, too.

Original languageEnglish
Pages (from-to)7937-7944
Number of pages8
JournalApplied Microbiology and Biotechnology
Volume99
Issue number19
DOIs
Publication statusPublished - Oct 10 2015

Keywords

  • Aspergillus terreus
  • Fermentation
  • Itaconic acid
  • Manganese ions
  • Specific yield
  • Volumetric yield
  • d-Glucose

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

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