A decade of aerobic endurance training

Histological evidence for fibre type transformation

R. Thayer, J. Collins, E. G. Noble, A. Taylor

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Background. Researchers employing a variety of training methods have demonstrated a fast-to-slow fibre transformation in animal skeletal muscle. The observation as to whether this occurs in exercise trained humans is limited and equivocal. Methods. Experimental design: to examine this issue, skeletal muscle from seven subjects who had participated in a decade or more of high intensity aerobic training (DT) and six nontrained (NT) subjects was obtained by muscle biopsy from the vastus lateralis muscle (VL) and subjected to a modified myofibrillar ATPase technique to identify muscle fibre types. Muscle tissue was histochemically treated by exposure to an alkaline preincubation (pH 9.9), an acid preincubation (pH 4.3 or 4.6) and the formate-KCl preincubation buffer (pH 4.54), previously employed in animal studies. Results. The formate-KCl preincubation medium identified all major fibre types at a single pH in human subjects. The percentage of type I fibres in DT was 70.9% vs 37.7% in NT (p2) was greater (p2) or II (5693.5±1214.6 μm2) from NT. Conclusions. The results revealed that endurance training may promote a transition from type II to type I muscle fibre types and occurs at the expense of the type II fibre population.

Original languageEnglish
Pages (from-to)284-289
Number of pages6
JournalJournal of Sports Medicine and Physical Fitness
Volume40
Issue number4
Publication statusPublished - 2000

Fingerprint

formic acid
Muscles
Skeletal Muscle
Slow-Twitch Muscle Fibers
Quadriceps Muscle
Adenosine Triphosphatases
Buffers
Research Design
Research Personnel
Observation
Biopsy
Acids
Population

Keywords

  • Adenosine triphosphatase metabolism
  • Cross sectional area
  • Muscle biopsy
  • Muscle fibre metabolism
  • Oxidative and glycolytic stain

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Physical Therapy, Sports Therapy and Rehabilitation
  • Orthopedics and Sports Medicine

Cite this

A decade of aerobic endurance training : Histological evidence for fibre type transformation. / Thayer, R.; Collins, J.; Noble, E. G.; Taylor, A.

In: Journal of Sports Medicine and Physical Fitness, Vol. 40, No. 4, 2000, p. 284-289.

Research output: Contribution to journalArticle

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