A convenient method for the synthesis of oligonucleotide-cationic peptide conjugates

G. Ferenc, Z. Kupihár, Z. Kele, L. Kovács

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

A method was developed for the synthesis of oligonucleotide-cationic peptide conjugates in solution phase by disulfide bond formation. Precipitation was avoided by the easily removable triethylammonium trifluoroacetate (TEATFAc) salt which served at the same time as a buffer of the reaction mixture. The fast and high yielding disulfide bond formation was due to the Npys thio protecting and activating group of Cys. A solution of the free 5′-thiol modified oligonucleotide obtained from Poly-Pak™ purification was used for conjugation.

Original languageEnglish
Pages (from-to)1059-1061
Number of pages3
JournalNucleosides, Nucleotides and Nucleic Acids
Volume24
Issue number5-7
DOIs
Publication statusPublished - 2005

Keywords

  • Cationic Peptide
  • Conjugation
  • Disulfide Bond Formation
  • Oligonucleotide
  • Penetration

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Genetics

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