A confocal microscopic evaluation of the effects of insulin imprinting on the binding of Concanavalin A by Tetrahymena pyriformis.

Peter Kovcs, Christine Sundermann, Barbara H. Estridge, G. Csaba

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

With confocal microscopy it is possible to study the Concanavalin A (Con A) binding characteristics of the surface and interior of a single cell by viewing optical sections. It was observed in Tetrahymena pyriformis that Con A bound both to the plasma membrane and to intracellular structures. Incubation of cells with a competing sugar a-methylmannopyranoside, decreased binding. Hormonal imprinting with insulin resulted in an increase in binding of Con A to the cell surface and a decrease in intracellular binding. It is possible that the intracellular binding sites may migrate to the plasma membrane.

Original languageEnglish
Pages (from-to)973-978
Number of pages6
JournalCell Biology International
Volume19
Issue number12
DOIs
Publication statusPublished - Dec 1995

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Tetrahymena pyriformis
Concanavalin A
Insulin
Methylmannosides
Cell Membrane
Confocal Microscopy
Binding Sites

ASJC Scopus subject areas

  • Cell Biology

Cite this

A confocal microscopic evaluation of the effects of insulin imprinting on the binding of Concanavalin A by Tetrahymena pyriformis. / Kovcs, Peter; Sundermann, Christine; Estridge, Barbara H.; Csaba, G.

In: Cell Biology International, Vol. 19, No. 12, 12.1995, p. 973-978.

Research output: Contribution to journalArticle

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