A confocal microscopic evaluation of the effects of insulin imprinting on the binding of Concanavalin A by Tetrahymena pyriformis.

Peter Kovcs, Christine Sundermann, Barbara H. Estridge, György Csaba

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Abstract

With confocal microscopy it is possible to study the Concanavalin A (Con A) binding characteristics of the surface and interior of a single cell by viewing optical sections. It was observed in Tetrahymena pyriformis that Con A bound both to the plasma membrane and to intracellular structures. Incubation of cells with a competing sugar a-methylmannopyranoside, decreased binding. Hormonal imprinting with insulin resulted in an increase in binding of Con A to the cell surface and a decrease in intracellular binding. It is possible that the intracellular binding sites may migrate to the plasma membrane.

Original languageEnglish
Pages (from-to)973-978
Number of pages6
JournalCell biology international
Volume19
Issue number12
DOIs
Publication statusPublished - Dec 1 1995

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ASJC Scopus subject areas

  • Cell Biology

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