A computer-assisted microscopic analysis of bone tissue developed inside a polyactive polymer implanted into an equine articular surface

Réka Albert, Gábor Vásárhelyi, Gábor Bodó, Annamária Kenyeres, Ervin Wolf, Tamás Papp, Tünde Terdik, László Módis, Szabolcs Felszeghy

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2 Citations (Scopus)

Abstract

One of the most promising applications for the restoration of small or moderately sized focal articular lesions is mosaicplasty (MP). Although recurrent hemarthrosis is a rare complication after MP, recently, various strategies have been designed to find an effective filling material to prevent postoperative bleeding from the donor site. The porous biodegradable polymer Polyactive (PA; a polyethylene glycol terephthalate - polybutylene terephthalate copolymer) represents a promising solution in this respect. A histological evaluation of the longterm PA-filled donor sites obtained from 10 experimental horses was performed. In this study, attention was primarily focused on the bone tissue developed in the plug. A computerassisted image analysis and quantitative polarized light microscopic measurements of decalcified, longitudinally sectioned, dimethylmethylene blue (DMMB)- and picrosirius red (PS) stained sections revealed that the coverage area of the bone trabecules in the PA-filled donor tunnels was substantially (25%) enlarged compared to the neighboring cancellous bone. For this quantification, identical ROIs (regions of interest) were used and compared. The birefringence retardation values were also measured with a polarized light microscope using monochromatic light. Identical retardation values could be recorded from the bone trabeculae developed in the PA and in the neighboring bone, which indicates that the collagen orientation pattern does not differ significantly among these bone trabecules. Based on our new data, we speculate that PA promotes bone formation, and some of the currently identified degradation products of PA may enhance osteoconduction and osteoinduction inside the donor canal.

Original languageEnglish
Pages (from-to)1203-1209
Number of pages7
JournalHistology and Histopathology
Volume27
Issue number9
Publication statusPublished - Sep 1 2012

Keywords

  • Bone
  • Computer-assisted image analysis
  • Donor tunnels
  • Horse
  • Mosaicplasty
  • Polarized light microscopy

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology

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  • Cite this

    Albert, R., Vásárhelyi, G., Bodó, G., Kenyeres, A., Wolf, E., Papp, T., Terdik, T., Módis, L., & Felszeghy, S. (2012). A computer-assisted microscopic analysis of bone tissue developed inside a polyactive polymer implanted into an equine articular surface. Histology and Histopathology, 27(9), 1203-1209.