A comprehensive assessment of apigenin as an antiproliferative, proapoptotic, antiangiogenic and immunomodulatory phytocompound

Alexandra Ghițu, Anja Schwiebs, Heinfried H. Radeke, Stefana Avram, I. Zupkó, Andrea Bor, Ioana Zinuca Pavel, Cristina Adriana Dehelean, Camelia Oprean, Florina Bojin, Claudia Farcas, Codruta Soica, Oana Duicu, Corina Danciu

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Apigenin (4′,5,7-trihydroxyflavone) (Api) is an important component of the human diet, being distributed in a wide number of fruits, vegetables and herbs with the most important sources being represented by chamomile, celery, celeriac and parsley. This study was designed for a comprehensive evaluation of Api as an antiproliferative, proapoptotic, antiangiogenic and immunomodulatory phytocompound. In the set experimental conditions, Api presents antiproliferative activity against the A375 human melanoma cell line, a G2/M arrest of the cell cycle and cytotoxic events as revealed by the lactate dehydrogenase release. Caspase 3 activity was inversely proportional to the Api tested doses, namely 30 μM and 60 μM. Phenomena of early apoptosis, late apoptosis and necrosis following incubation with Api were detected by Annexin V-PI double staining. The flavone interfered with the mitochondrial respiration by modulating both glycolytic and mitochondrial pathways for ATP production. The metabolic activity of human dendritic cells (DCs) under LPS-activation was clearly attenuated by stimulation with high concentrations of Api. Il-6 and IL-10 secretion was almost completely blocked while TNF alpha secretion was reduced by about 60%. Api elicited antiangiogenic properties in a dose-dependent manner. Both concentrations of Api influenced tumour cell growth and migration, inducing a limited tumour area inside the application ring, associated with a low number of capillaries.

Original languageEnglish
Article number858
JournalNutrients
Volume11
Issue number4
DOIs
Publication statusPublished - Apr 1 2019

    Fingerprint

Keywords

  • A375 human melanoma cell line
  • Angiogenesis
  • Apigenin
  • Apoptosis
  • Mitochondrial bioenergetics and glycolysis
  • Proliferation

ASJC Scopus subject areas

  • Food Science
  • Nutrition and Dietetics

Cite this

Ghițu, A., Schwiebs, A., Radeke, H. H., Avram, S., Zupkó, I., Bor, A., Pavel, I. Z., Dehelean, C. A., Oprean, C., Bojin, F., Farcas, C., Soica, C., Duicu, O., & Danciu, C. (2019). A comprehensive assessment of apigenin as an antiproliferative, proapoptotic, antiangiogenic and immunomodulatory phytocompound. Nutrients, 11(4), [858]. https://doi.org/10.3390/nu11040858