Abstract
In the quest for new drugs of herbal origin, the ethyl acetate (EAE), methanol (ME), and water (WE) extracts of Crocus chrysanthus (Herb.) Herb. flowers were analyzed for their polyphenolic composition, antioxidant, and enzyme inhibitory potential. WE showed the highest antioxidant activities in all assays including metal chelating, phosphomolybdenum, FRAP, CUPRAC, ABTS and DPPH. EAE was the most effective enzyme inhibitor, exhibiting the highest inhibition against some enzymes linked to Alzheimer's disease (cholinesterases), diabetes mellitus (α-glucosidase and α-amylase) and hyperpigmentation problems (tyrosinase). The highest total phenolics (34.99 mg GAE/g) and flavonoids content (77.58 mg RE/g) were observed in WE and ME, respectively. Eight compounds were identified in EAE, 24 in ME, and 15 in WE. Kaempferol 3-O glucoside was found in all extracts. In conclusion, C. chrysanthus flowers can be suggested as a source of bioactive components with potential use against chronic disorders caused by oxidative stress. Future in-depth studies are recommended to determine the biological effects of isolated compounds from C. chrysanthus to identify the main compounds modulating the observed activities.
| Language | English |
|---|---|
| Pages | 581-589 |
| Number of pages | 9 |
| Journal | Journal of Pharmaceutical and Biomedical Analysis |
| Volume | 164 |
| DOIs | |
| Publication status | Published - Feb 5 2019 |
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Keywords
- Crocus chrysanthus
- Enzyme inhibitor
- Oxidative stress
- Polyphenolic
ASJC Scopus subject areas
- Analytical Chemistry
- Pharmaceutical Science
- Drug Discovery
- Spectroscopy
- Clinical Biochemistry
Cite this
A comprehensive appraisal on Crocus chrysanthus (Herb.) Herb. flower extracts with HPLC–MS/MS profiles, antioxidant and enzyme inhibitory properties. / Zengin, Gokhan; Aumeeruddy, Muhammad Zakariyyah; Diuzheva, Alina; Jekó́, J.; Cziáky, Zoltán; Yıldıztugay, Aysegul; Yıldıztugay, Evren; Mahomoodally, Mohamad Fawzi.
In: Journal of Pharmaceutical and Biomedical Analysis, Vol. 164, 05.02.2019, p. 581-589.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A comprehensive appraisal on Crocus chrysanthus (Herb.) Herb. flower extracts with HPLC–MS/MS profiles, antioxidant and enzyme inhibitory properties
AU - Zengin, Gokhan
AU - Aumeeruddy, Muhammad Zakariyyah
AU - Diuzheva, Alina
AU - Jekó́, J.
AU - Cziáky, Zoltán
AU - Yıldıztugay, Aysegul
AU - Yıldıztugay, Evren
AU - Mahomoodally, Mohamad Fawzi
PY - 2019/2/5
Y1 - 2019/2/5
N2 - In the quest for new drugs of herbal origin, the ethyl acetate (EAE), methanol (ME), and water (WE) extracts of Crocus chrysanthus (Herb.) Herb. flowers were analyzed for their polyphenolic composition, antioxidant, and enzyme inhibitory potential. WE showed the highest antioxidant activities in all assays including metal chelating, phosphomolybdenum, FRAP, CUPRAC, ABTS and DPPH. EAE was the most effective enzyme inhibitor, exhibiting the highest inhibition against some enzymes linked to Alzheimer's disease (cholinesterases), diabetes mellitus (α-glucosidase and α-amylase) and hyperpigmentation problems (tyrosinase). The highest total phenolics (34.99 mg GAE/g) and flavonoids content (77.58 mg RE/g) were observed in WE and ME, respectively. Eight compounds were identified in EAE, 24 in ME, and 15 in WE. Kaempferol 3-O glucoside was found in all extracts. In conclusion, C. chrysanthus flowers can be suggested as a source of bioactive components with potential use against chronic disorders caused by oxidative stress. Future in-depth studies are recommended to determine the biological effects of isolated compounds from C. chrysanthus to identify the main compounds modulating the observed activities.
AB - In the quest for new drugs of herbal origin, the ethyl acetate (EAE), methanol (ME), and water (WE) extracts of Crocus chrysanthus (Herb.) Herb. flowers were analyzed for their polyphenolic composition, antioxidant, and enzyme inhibitory potential. WE showed the highest antioxidant activities in all assays including metal chelating, phosphomolybdenum, FRAP, CUPRAC, ABTS and DPPH. EAE was the most effective enzyme inhibitor, exhibiting the highest inhibition against some enzymes linked to Alzheimer's disease (cholinesterases), diabetes mellitus (α-glucosidase and α-amylase) and hyperpigmentation problems (tyrosinase). The highest total phenolics (34.99 mg GAE/g) and flavonoids content (77.58 mg RE/g) were observed in WE and ME, respectively. Eight compounds were identified in EAE, 24 in ME, and 15 in WE. Kaempferol 3-O glucoside was found in all extracts. In conclusion, C. chrysanthus flowers can be suggested as a source of bioactive components with potential use against chronic disorders caused by oxidative stress. Future in-depth studies are recommended to determine the biological effects of isolated compounds from C. chrysanthus to identify the main compounds modulating the observed activities.
KW - Crocus chrysanthus
KW - Enzyme inhibitor
KW - Oxidative stress
KW - Polyphenolic
UR - http://www.scopus.com/inward/record.url?scp=85056733063&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85056733063&partnerID=8YFLogxK
U2 - 10.1016/j.jpba.2018.11.022
DO - 10.1016/j.jpba.2018.11.022
M3 - Article
VL - 164
SP - 581
EP - 589
JO - Journal of Pharmaceutical and Biomedical Analysis
T2 - Journal of Pharmaceutical and Biomedical Analysis
JF - Journal of Pharmaceutical and Biomedical Analysis
SN - 0731-7085
ER -