A comparison of techniques for size measurement of nanoparticles in cell culture medium

Christian Gollwitzer, Dorota Bartczak, Heidi Goenaga-Infante, Vikram Kestens, Michael Krumrey, Caterina Minelli, Marcell Pálmai, Yannic Ramaye, Gert Roebben, Aneta Sikora, Z. Varga

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Plain and aminated silica nanoparticles dispersed in purified water, in 50 mM Tris-HCl buffer and in cell culture medium were measured using dynamic light scattering (DLS), centrifugal liquid sedimentation (CLS), small-angle X-ray scattering (SAXS), and particle tracking analysis (PTA). The test samples were measured by all methods immediately after dispersion and after incubation at room temperature for 24 h. The effect of the biological dispersion medium on the modal value of the particle size distribution was compared for each method taking into account the estimated uncertainty. For the methods based on light scattering, DLS and PTA, the size distributions obtained were significantly altered due to the formation of a protein corona and induced agglomeration effects. With SAXS and CLS, the measured size of the primary particles was mostly unchanged. While SAXS offers excellent precision and traceability to the SI unit system if the model fitting approach is used for data analysis, CLS provides detailed size distributions from which additional information on the agglomeration state can be deduced.

Original languageEnglish
Pages (from-to)5272-5282
Number of pages11
JournalAnalytical Methods
Volume8
Issue number26
DOIs
Publication statusPublished - Jul 14 2016

Fingerprint

X ray scattering
Sedimentation
Cell culture
Culture Media
Dynamic light scattering
Nanoparticles
Liquids
Agglomeration
Particle size analysis
Silicon Dioxide
Light scattering
Buffers
Silica
Proteins
Water
Temperature

ASJC Scopus subject areas

  • Analytical Chemistry
  • Engineering(all)
  • Chemical Engineering(all)

Cite this

Gollwitzer, C., Bartczak, D., Goenaga-Infante, H., Kestens, V., Krumrey, M., Minelli, C., ... Varga, Z. (2016). A comparison of techniques for size measurement of nanoparticles in cell culture medium. Analytical Methods, 8(26), 5272-5282. https://doi.org/10.1039/c6ay00419a

A comparison of techniques for size measurement of nanoparticles in cell culture medium. / Gollwitzer, Christian; Bartczak, Dorota; Goenaga-Infante, Heidi; Kestens, Vikram; Krumrey, Michael; Minelli, Caterina; Pálmai, Marcell; Ramaye, Yannic; Roebben, Gert; Sikora, Aneta; Varga, Z.

In: Analytical Methods, Vol. 8, No. 26, 14.07.2016, p. 5272-5282.

Research output: Contribution to journalArticle

Gollwitzer, C, Bartczak, D, Goenaga-Infante, H, Kestens, V, Krumrey, M, Minelli, C, Pálmai, M, Ramaye, Y, Roebben, G, Sikora, A & Varga, Z 2016, 'A comparison of techniques for size measurement of nanoparticles in cell culture medium', Analytical Methods, vol. 8, no. 26, pp. 5272-5282. https://doi.org/10.1039/c6ay00419a
Gollwitzer C, Bartczak D, Goenaga-Infante H, Kestens V, Krumrey M, Minelli C et al. A comparison of techniques for size measurement of nanoparticles in cell culture medium. Analytical Methods. 2016 Jul 14;8(26):5272-5282. https://doi.org/10.1039/c6ay00419a
Gollwitzer, Christian ; Bartczak, Dorota ; Goenaga-Infante, Heidi ; Kestens, Vikram ; Krumrey, Michael ; Minelli, Caterina ; Pálmai, Marcell ; Ramaye, Yannic ; Roebben, Gert ; Sikora, Aneta ; Varga, Z. / A comparison of techniques for size measurement of nanoparticles in cell culture medium. In: Analytical Methods. 2016 ; Vol. 8, No. 26. pp. 5272-5282.
@article{0a2f79043c5d46419faba0f9c6f9f0ce,
title = "A comparison of techniques for size measurement of nanoparticles in cell culture medium",
abstract = "Plain and aminated silica nanoparticles dispersed in purified water, in 50 mM Tris-HCl buffer and in cell culture medium were measured using dynamic light scattering (DLS), centrifugal liquid sedimentation (CLS), small-angle X-ray scattering (SAXS), and particle tracking analysis (PTA). The test samples were measured by all methods immediately after dispersion and after incubation at room temperature for 24 h. The effect of the biological dispersion medium on the modal value of the particle size distribution was compared for each method taking into account the estimated uncertainty. For the methods based on light scattering, DLS and PTA, the size distributions obtained were significantly altered due to the formation of a protein corona and induced agglomeration effects. With SAXS and CLS, the measured size of the primary particles was mostly unchanged. While SAXS offers excellent precision and traceability to the SI unit system if the model fitting approach is used for data analysis, CLS provides detailed size distributions from which additional information on the agglomeration state can be deduced.",
author = "Christian Gollwitzer and Dorota Bartczak and Heidi Goenaga-Infante and Vikram Kestens and Michael Krumrey and Caterina Minelli and Marcell P{\'a}lmai and Yannic Ramaye and Gert Roebben and Aneta Sikora and Z. Varga",
year = "2016",
month = "7",
day = "14",
doi = "10.1039/c6ay00419a",
language = "English",
volume = "8",
pages = "5272--5282",
journal = "Analytical Methods",
issn = "1759-9660",
publisher = "Royal Society of Chemistry",
number = "26",

}

TY - JOUR

T1 - A comparison of techniques for size measurement of nanoparticles in cell culture medium

AU - Gollwitzer, Christian

AU - Bartczak, Dorota

AU - Goenaga-Infante, Heidi

AU - Kestens, Vikram

AU - Krumrey, Michael

AU - Minelli, Caterina

AU - Pálmai, Marcell

AU - Ramaye, Yannic

AU - Roebben, Gert

AU - Sikora, Aneta

AU - Varga, Z.

PY - 2016/7/14

Y1 - 2016/7/14

N2 - Plain and aminated silica nanoparticles dispersed in purified water, in 50 mM Tris-HCl buffer and in cell culture medium were measured using dynamic light scattering (DLS), centrifugal liquid sedimentation (CLS), small-angle X-ray scattering (SAXS), and particle tracking analysis (PTA). The test samples were measured by all methods immediately after dispersion and after incubation at room temperature for 24 h. The effect of the biological dispersion medium on the modal value of the particle size distribution was compared for each method taking into account the estimated uncertainty. For the methods based on light scattering, DLS and PTA, the size distributions obtained were significantly altered due to the formation of a protein corona and induced agglomeration effects. With SAXS and CLS, the measured size of the primary particles was mostly unchanged. While SAXS offers excellent precision and traceability to the SI unit system if the model fitting approach is used for data analysis, CLS provides detailed size distributions from which additional information on the agglomeration state can be deduced.

AB - Plain and aminated silica nanoparticles dispersed in purified water, in 50 mM Tris-HCl buffer and in cell culture medium were measured using dynamic light scattering (DLS), centrifugal liquid sedimentation (CLS), small-angle X-ray scattering (SAXS), and particle tracking analysis (PTA). The test samples were measured by all methods immediately after dispersion and after incubation at room temperature for 24 h. The effect of the biological dispersion medium on the modal value of the particle size distribution was compared for each method taking into account the estimated uncertainty. For the methods based on light scattering, DLS and PTA, the size distributions obtained were significantly altered due to the formation of a protein corona and induced agglomeration effects. With SAXS and CLS, the measured size of the primary particles was mostly unchanged. While SAXS offers excellent precision and traceability to the SI unit system if the model fitting approach is used for data analysis, CLS provides detailed size distributions from which additional information on the agglomeration state can be deduced.

UR - http://www.scopus.com/inward/record.url?scp=84977134315&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84977134315&partnerID=8YFLogxK

U2 - 10.1039/c6ay00419a

DO - 10.1039/c6ay00419a

M3 - Article

VL - 8

SP - 5272

EP - 5282

JO - Analytical Methods

JF - Analytical Methods

SN - 1759-9660

IS - 26

ER -