A Comparison of 1D and 2D (Unbiased) Experimental Methods for Measuring CSA/DD Cross-Correlated Relaxation

G. Batta, K. Kövér, J. Kowalewski

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Conventional and enhanced 1D experiments and different NOESY experiments (the 2D unbiased method) were performed for measuring CSA/DD cross-correlated relaxation on trehalose, a compound which could be approximated as a spherical top, and on simple model compounds comprising C3v symmetry (CHCl3, triphenylsilane (TPSi)). The comparison gives experimental evidence for the equivalence of the methods within the limits of the two-spin approach. 1D data are evaluated with both the simple initial rate and the Redfield relaxation matrix approach. The 2D data are obtained from the so-called transfer matrix using the Perrin-Gipe eigenvalue/eigenvector method. For the improved performance of the 2D method, an X-filtered (HHH) NOESY is suggested at the natural abundance of 13C (or other dilute, low γ species). Also, experimental parameters crucial for reliable CSA data are tested (e.g., the impact of insufficient relaxation delay). Error estimation is carried out for fair comparison of methods. Revised liquid state 1H and 13C (29Si) CSA data are presented for chloroform and TPSi.

Original languageEnglish
Pages (from-to)37-46
Number of pages10
JournalJournal of Magnetic Resonance
Volume136
Issue number1
Publication statusPublished - Jan 1999

Fingerprint

cross relaxation
Trehalose
Chloroform
chloroform
Eigenvalues and eigenfunctions
Error analysis
equivalence
eigenvectors
eigenvalues
Experiments
Liquids
symmetry
matrices
liquids

Keywords

  • 1D techniques
  • Chloroform
  • CSA/DD cross-correlated relaxation
  • Triphenylsilane
  • Unbiased method

ASJC Scopus subject areas

  • Molecular Biology
  • Physical and Theoretical Chemistry
  • Spectroscopy
  • Radiology Nuclear Medicine and imaging
  • Condensed Matter Physics

Cite this

A Comparison of 1D and 2D (Unbiased) Experimental Methods for Measuring CSA/DD Cross-Correlated Relaxation. / Batta, G.; Kövér, K.; Kowalewski, J.

In: Journal of Magnetic Resonance, Vol. 136, No. 1, 01.1999, p. 37-46.

Research output: Contribution to journalArticle

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