A comparative study on the adenine nucleotide metabolism of acid-dependent and non-acid-dependent acute gastric mucosal injury in the rat

B. Gasztonyi, A. Kiraly, G. Sütö, A. Vincze, O. Karadi, G. Mózsik

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Abstract

Intragastric (ig) administration of 96% ethanol (EtOH, non-acid-dependent model) and 0.6 mol/L HCl (acid-dependent model) produces acute mucosal lesions in the rat stomach. Aim: The aim of this study was to compare the EtOH- and HCl-induced biochemical changes and ulcerations of gastric mucosa in the rat examined at different times after administration of the necrotizing agents. Materials and methods: The observations were carried out on Sprague-Dawley rats weighing 180-210 g. The animals were fasted for 24 h before the experiment but received water freely; 1 ml 96% EtOH or 0.6 mol/L HCl was given ig. The rats were sacrificed at 0, 1, 5, 15, 30 and 60 min after administration of necrotizing agents, when the stomach was removed and the number and severity of mucosal lesions was noted. The mucosa was scraped and its ATP, ADP, AMP and lactate levels were measured enzymatically; the cAMP level was measured by RIA. The ratio of ATP/ADP, adenylate pool (ATP+ADP+AMP) and 'energy charge' [(ATP+0.5ADP)/(ATP+ADP+AMP)] were calculated. Results: 1. The number and severity of mucosal lesions reached about 50% of total by 5 min after application of the necrotizing agents; 2. The ATP level decreased between 5 and 30 min, but thereafter its level increased (ADP changed in the opposite direction) in both models; 3. The cAMP level increased at 1 and 5 min but decreased later; 4. 'Energy charge' and lactate level did not change. Conclusions: The biochemical changes in acid- (HCl) and non-acid- (EtOH) dependent gastric ulcer models are similar, but these changes appear earlier in the acid-dependent model.

Original languageEnglish
Pages (from-to)351-360
Number of pages10
JournalInflammopharmacology
Volume4
Issue number4
Publication statusPublished - 1996

Fingerprint

Adenine Nucleotides
Stomach
Adenosine Diphosphate
Adenosine Triphosphate
Acids
Wounds and Injuries
Adenosine Monophosphate
Lactic Acid
Stomach Ulcer
Gastric Mucosa
Sprague Dawley Rats
Mucous Membrane
Ethanol
Water

Keywords

  • acid-dependent model
  • cellular energy systems
  • non-acid-dependent models

ASJC Scopus subject areas

  • Immunology
  • Pharmacology

Cite this

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title = "A comparative study on the adenine nucleotide metabolism of acid-dependent and non-acid-dependent acute gastric mucosal injury in the rat",
abstract = "Intragastric (ig) administration of 96{\%} ethanol (EtOH, non-acid-dependent model) and 0.6 mol/L HCl (acid-dependent model) produces acute mucosal lesions in the rat stomach. Aim: The aim of this study was to compare the EtOH- and HCl-induced biochemical changes and ulcerations of gastric mucosa in the rat examined at different times after administration of the necrotizing agents. Materials and methods: The observations were carried out on Sprague-Dawley rats weighing 180-210 g. The animals were fasted for 24 h before the experiment but received water freely; 1 ml 96{\%} EtOH or 0.6 mol/L HCl was given ig. The rats were sacrificed at 0, 1, 5, 15, 30 and 60 min after administration of necrotizing agents, when the stomach was removed and the number and severity of mucosal lesions was noted. The mucosa was scraped and its ATP, ADP, AMP and lactate levels were measured enzymatically; the cAMP level was measured by RIA. The ratio of ATP/ADP, adenylate pool (ATP+ADP+AMP) and 'energy charge' [(ATP+0.5ADP)/(ATP+ADP+AMP)] were calculated. Results: 1. The number and severity of mucosal lesions reached about 50{\%} of total by 5 min after application of the necrotizing agents; 2. The ATP level decreased between 5 and 30 min, but thereafter its level increased (ADP changed in the opposite direction) in both models; 3. The cAMP level increased at 1 and 5 min but decreased later; 4. 'Energy charge' and lactate level did not change. Conclusions: The biochemical changes in acid- (HCl) and non-acid- (EtOH) dependent gastric ulcer models are similar, but these changes appear earlier in the acid-dependent model.",
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year = "1996",
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T1 - A comparative study on the adenine nucleotide metabolism of acid-dependent and non-acid-dependent acute gastric mucosal injury in the rat

AU - Gasztonyi, B.

AU - Kiraly, A.

AU - Sütö, G.

AU - Vincze, A.

AU - Karadi, O.

AU - Mózsik, G.

PY - 1996

Y1 - 1996

N2 - Intragastric (ig) administration of 96% ethanol (EtOH, non-acid-dependent model) and 0.6 mol/L HCl (acid-dependent model) produces acute mucosal lesions in the rat stomach. Aim: The aim of this study was to compare the EtOH- and HCl-induced biochemical changes and ulcerations of gastric mucosa in the rat examined at different times after administration of the necrotizing agents. Materials and methods: The observations were carried out on Sprague-Dawley rats weighing 180-210 g. The animals were fasted for 24 h before the experiment but received water freely; 1 ml 96% EtOH or 0.6 mol/L HCl was given ig. The rats were sacrificed at 0, 1, 5, 15, 30 and 60 min after administration of necrotizing agents, when the stomach was removed and the number and severity of mucosal lesions was noted. The mucosa was scraped and its ATP, ADP, AMP and lactate levels were measured enzymatically; the cAMP level was measured by RIA. The ratio of ATP/ADP, adenylate pool (ATP+ADP+AMP) and 'energy charge' [(ATP+0.5ADP)/(ATP+ADP+AMP)] were calculated. Results: 1. The number and severity of mucosal lesions reached about 50% of total by 5 min after application of the necrotizing agents; 2. The ATP level decreased between 5 and 30 min, but thereafter its level increased (ADP changed in the opposite direction) in both models; 3. The cAMP level increased at 1 and 5 min but decreased later; 4. 'Energy charge' and lactate level did not change. Conclusions: The biochemical changes in acid- (HCl) and non-acid- (EtOH) dependent gastric ulcer models are similar, but these changes appear earlier in the acid-dependent model.

AB - Intragastric (ig) administration of 96% ethanol (EtOH, non-acid-dependent model) and 0.6 mol/L HCl (acid-dependent model) produces acute mucosal lesions in the rat stomach. Aim: The aim of this study was to compare the EtOH- and HCl-induced biochemical changes and ulcerations of gastric mucosa in the rat examined at different times after administration of the necrotizing agents. Materials and methods: The observations were carried out on Sprague-Dawley rats weighing 180-210 g. The animals were fasted for 24 h before the experiment but received water freely; 1 ml 96% EtOH or 0.6 mol/L HCl was given ig. The rats were sacrificed at 0, 1, 5, 15, 30 and 60 min after administration of necrotizing agents, when the stomach was removed and the number and severity of mucosal lesions was noted. The mucosa was scraped and its ATP, ADP, AMP and lactate levels were measured enzymatically; the cAMP level was measured by RIA. The ratio of ATP/ADP, adenylate pool (ATP+ADP+AMP) and 'energy charge' [(ATP+0.5ADP)/(ATP+ADP+AMP)] were calculated. Results: 1. The number and severity of mucosal lesions reached about 50% of total by 5 min after application of the necrotizing agents; 2. The ATP level decreased between 5 and 30 min, but thereafter its level increased (ADP changed in the opposite direction) in both models; 3. The cAMP level increased at 1 and 5 min but decreased later; 4. 'Energy charge' and lactate level did not change. Conclusions: The biochemical changes in acid- (HCl) and non-acid- (EtOH) dependent gastric ulcer models are similar, but these changes appear earlier in the acid-dependent model.

KW - acid-dependent model

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