A comparative study of the inhibitory effect of trypan blue on mouse erythrocyte and C3 binding receptors of peripheral blood mononuclear cells from healthy donors and patients suffering from chronic lymphocytic leukaemia.

A. Dobozy, J. Hunyadi, A. S. Kenderessy, F. Krizsa, K. Boda, N. Simon

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Trypan blue has previously been shown to interact with the C3 receptor, but not with the Fc receptor. In the present work the effects of Trypan blue on mouse erythrocyte (ME) and C3 binding receptors of the peripheral blood mononuclear cells have been studied in healthy individuals and in patients with chronic lymphocytic leukemia (CLL). The resulting dose-response curves have been compared with each another. The results of statistical analysis indicate that there is no significant difference between the effects of trypan blue on the two receptors in healthy individuals. In contrast ME and C3 binding receptors differ significantly in trypan blue sensitivity in CLL patients. These data suggest that the two receptors are similar, or are situated on the cell membrane in such a way that the trypan blue bound to one of them inhibits the functioning of the other one too, but they are not the same. In the course of leukemic transformation, the trypan blue sensitivity of the ME binding receptor does not vary, whereas that of the C3 receptor is enhanced significantly.

Original languageEnglish
Pages (from-to)510-516
Number of pages7
JournalFolia haematologica : internationales Magazin fur klinische und morphologische Blutforschung
Volume110
Issue number4
Publication statusPublished - 1983

Fingerprint

Trypan Blue
B-Cell Chronic Lymphocytic Leukemia
Blood Cells
Erythrocytes
Tissue Donors
Fc Receptors
Cell Membrane

ASJC Scopus subject areas

  • Medicine(all)

Cite this

@article{737c0dcd650a4e619a3c5f1acac474bf,
title = "A comparative study of the inhibitory effect of trypan blue on mouse erythrocyte and C3 binding receptors of peripheral blood mononuclear cells from healthy donors and patients suffering from chronic lymphocytic leukaemia.",
abstract = "Trypan blue has previously been shown to interact with the C3 receptor, but not with the Fc receptor. In the present work the effects of Trypan blue on mouse erythrocyte (ME) and C3 binding receptors of the peripheral blood mononuclear cells have been studied in healthy individuals and in patients with chronic lymphocytic leukemia (CLL). The resulting dose-response curves have been compared with each another. The results of statistical analysis indicate that there is no significant difference between the effects of trypan blue on the two receptors in healthy individuals. In contrast ME and C3 binding receptors differ significantly in trypan blue sensitivity in CLL patients. These data suggest that the two receptors are similar, or are situated on the cell membrane in such a way that the trypan blue bound to one of them inhibits the functioning of the other one too, but they are not the same. In the course of leukemic transformation, the trypan blue sensitivity of the ME binding receptor does not vary, whereas that of the C3 receptor is enhanced significantly.",
author = "A. Dobozy and J. Hunyadi and Kenderessy, {A. S.} and F. Krizsa and K. Boda and N. Simon",
year = "1983",
language = "English",
volume = "110",
pages = "510--516",
journal = "Folia haematologica; internationales Magazin fur Blutforschung",
issn = "0015-556X",
publisher = "Akademische Verlagsgesellschaft",
number = "4",

}

TY - JOUR

T1 - A comparative study of the inhibitory effect of trypan blue on mouse erythrocyte and C3 binding receptors of peripheral blood mononuclear cells from healthy donors and patients suffering from chronic lymphocytic leukaemia.

AU - Dobozy, A.

AU - Hunyadi, J.

AU - Kenderessy, A. S.

AU - Krizsa, F.

AU - Boda, K.

AU - Simon, N.

PY - 1983

Y1 - 1983

N2 - Trypan blue has previously been shown to interact with the C3 receptor, but not with the Fc receptor. In the present work the effects of Trypan blue on mouse erythrocyte (ME) and C3 binding receptors of the peripheral blood mononuclear cells have been studied in healthy individuals and in patients with chronic lymphocytic leukemia (CLL). The resulting dose-response curves have been compared with each another. The results of statistical analysis indicate that there is no significant difference between the effects of trypan blue on the two receptors in healthy individuals. In contrast ME and C3 binding receptors differ significantly in trypan blue sensitivity in CLL patients. These data suggest that the two receptors are similar, or are situated on the cell membrane in such a way that the trypan blue bound to one of them inhibits the functioning of the other one too, but they are not the same. In the course of leukemic transformation, the trypan blue sensitivity of the ME binding receptor does not vary, whereas that of the C3 receptor is enhanced significantly.

AB - Trypan blue has previously been shown to interact with the C3 receptor, but not with the Fc receptor. In the present work the effects of Trypan blue on mouse erythrocyte (ME) and C3 binding receptors of the peripheral blood mononuclear cells have been studied in healthy individuals and in patients with chronic lymphocytic leukemia (CLL). The resulting dose-response curves have been compared with each another. The results of statistical analysis indicate that there is no significant difference between the effects of trypan blue on the two receptors in healthy individuals. In contrast ME and C3 binding receptors differ significantly in trypan blue sensitivity in CLL patients. These data suggest that the two receptors are similar, or are situated on the cell membrane in such a way that the trypan blue bound to one of them inhibits the functioning of the other one too, but they are not the same. In the course of leukemic transformation, the trypan blue sensitivity of the ME binding receptor does not vary, whereas that of the C3 receptor is enhanced significantly.

UR - http://www.scopus.com/inward/record.url?scp=0020992804&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0020992804&partnerID=8YFLogxK

M3 - Article

C2 - 6196267

AN - SCOPUS:0020992804

VL - 110

SP - 510

EP - 516

JO - Folia haematologica; internationales Magazin fur Blutforschung

JF - Folia haematologica; internationales Magazin fur Blutforschung

SN - 0015-556X

IS - 4

ER -