A comparative study of capillary zone electrophoresis and pH-potentiometry for determination of dissociation constants

Melinda Andrasi, P. Buglyó, Laszlo Zekany, A. Gáspár

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Acidity constants of six cephalosporin antibiotics, cefalexin, cefaclor, cefadroxil, cefotaxim, cefoperazon and cefoxitin are determined using capillary zone electrophoresis (CZE) and pH-potentiometric titrations. Since CZE is a separation method, it is not necessary for the samples to be of high purity and known concentration because only mobilities are measured. The effect on determination of dissociation constants of different matrices (serum, 0.9% NaCl, fermentation matrix) was examined. The advantages of CZE can be utilized in those fields where potentiometry has limitations (sample quantity, solubility, purity, simultaneous determinations), although pKa values that are close to each other can be determined by potentiometry with more accuracy.

Original languageEnglish
Pages (from-to)1040-1047
Number of pages8
JournalJournal of Pharmaceutical and Biomedical Analysis
Volume44
Issue number5
DOIs
Publication statusPublished - Sep 3 2007

Fingerprint

Potentiometry
Capillary Electrophoresis
Electrophoresis
Cefadroxil
Cefaclor
Cephalexin
Cefoperazone
Cefoxitin
Cefotaxime
Cephalosporins
Titration
Acidity
Solubility
Fermentation
Serum

Keywords

  • Cephalosporins
  • CZE
  • pH-potentiometry
  • pK determination

ASJC Scopus subject areas

  • Analytical Chemistry
  • Pharmaceutical Science

Cite this

A comparative study of capillary zone electrophoresis and pH-potentiometry for determination of dissociation constants. / Andrasi, Melinda; Buglyó, P.; Zekany, Laszlo; Gáspár, A.

In: Journal of Pharmaceutical and Biomedical Analysis, Vol. 44, No. 5, 03.09.2007, p. 1040-1047.

Research output: Contribution to journalArticle

@article{516ef39f497342d2873fdd00e3430674,
title = "A comparative study of capillary zone electrophoresis and pH-potentiometry for determination of dissociation constants",
abstract = "Acidity constants of six cephalosporin antibiotics, cefalexin, cefaclor, cefadroxil, cefotaxim, cefoperazon and cefoxitin are determined using capillary zone electrophoresis (CZE) and pH-potentiometric titrations. Since CZE is a separation method, it is not necessary for the samples to be of high purity and known concentration because only mobilities are measured. The effect on determination of dissociation constants of different matrices (serum, 0.9{\%} NaCl, fermentation matrix) was examined. The advantages of CZE can be utilized in those fields where potentiometry has limitations (sample quantity, solubility, purity, simultaneous determinations), although pKa values that are close to each other can be determined by potentiometry with more accuracy.",
keywords = "Cephalosporins, CZE, pH-potentiometry, pK determination",
author = "Melinda Andrasi and P. Bugly{\'o} and Laszlo Zekany and A. G{\'a}sp{\'a}r",
year = "2007",
month = "9",
day = "3",
doi = "10.1016/j.jpba.2007.04.024",
language = "English",
volume = "44",
pages = "1040--1047",
journal = "Journal of Pharmaceutical and Biomedical Analysis",
issn = "0731-7085",
publisher = "Elsevier",
number = "5",

}

TY - JOUR

T1 - A comparative study of capillary zone electrophoresis and pH-potentiometry for determination of dissociation constants

AU - Andrasi, Melinda

AU - Buglyó, P.

AU - Zekany, Laszlo

AU - Gáspár, A.

PY - 2007/9/3

Y1 - 2007/9/3

N2 - Acidity constants of six cephalosporin antibiotics, cefalexin, cefaclor, cefadroxil, cefotaxim, cefoperazon and cefoxitin are determined using capillary zone electrophoresis (CZE) and pH-potentiometric titrations. Since CZE is a separation method, it is not necessary for the samples to be of high purity and known concentration because only mobilities are measured. The effect on determination of dissociation constants of different matrices (serum, 0.9% NaCl, fermentation matrix) was examined. The advantages of CZE can be utilized in those fields where potentiometry has limitations (sample quantity, solubility, purity, simultaneous determinations), although pKa values that are close to each other can be determined by potentiometry with more accuracy.

AB - Acidity constants of six cephalosporin antibiotics, cefalexin, cefaclor, cefadroxil, cefotaxim, cefoperazon and cefoxitin are determined using capillary zone electrophoresis (CZE) and pH-potentiometric titrations. Since CZE is a separation method, it is not necessary for the samples to be of high purity and known concentration because only mobilities are measured. The effect on determination of dissociation constants of different matrices (serum, 0.9% NaCl, fermentation matrix) was examined. The advantages of CZE can be utilized in those fields where potentiometry has limitations (sample quantity, solubility, purity, simultaneous determinations), although pKa values that are close to each other can be determined by potentiometry with more accuracy.

KW - Cephalosporins

KW - CZE

KW - pH-potentiometry

KW - pK determination

UR - http://www.scopus.com/inward/record.url?scp=34547792223&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34547792223&partnerID=8YFLogxK

U2 - 10.1016/j.jpba.2007.04.024

DO - 10.1016/j.jpba.2007.04.024

M3 - Article

VL - 44

SP - 1040

EP - 1047

JO - Journal of Pharmaceutical and Biomedical Analysis

JF - Journal of Pharmaceutical and Biomedical Analysis

SN - 0731-7085

IS - 5

ER -