A comparative biomarker study of 514 matched cases of male and female breast cancer reveals gender-specific biological differences

Abeer M. Shaaban, Graham R. Ball, Rebecca A. Brannan, Gabor Cserni, Anna Di Benedetto, Jo Dent, Laura Fulford, Helen Honarpisheh, Lee Jordan, J. Louise Jones, Rani Kanthan, Loaie Maraqa, Maria Litwiniuk, Marcella Mottolese, Steven Pollock, Elena Provenzano, Philip R. Quinlan, Georgina Reall, Sami Shousha, Mark StephensEldo T. Verghese, Rosemary A. Walker, Andrew M. Hanby, Valerie Speirs

Research output: Contribution to journalArticle

75 Citations (Scopus)

Abstract

Male breast cancer remains understudied despite evidence of rising incidence. Using a co-ordinated multi-centre approach, we present the first large scale biomarker study to define and compare hormone receptor profiles and survival between male and female invasive breast cancer. We defined and compared hormone receptor profiles and survival between 251 male and 263 female breast cancers matched for grade, age, and lymph node status. Tissue microarrays were immunostained for ERα, ERβ1, -2, -5, PR, PRA, PRB and AR, augmented by HER2, CK5/6, 14, 18 and 19 to assist typing. Hierarchical clustering determined differential nature of influences between genders. Luminal A was the most common phe-notype in both sexes. Luminal B and HER2 were not seen in males. Basal phenotype was infrequent in both. No differences in overall survival at 5 or 10 years were observed between genders. Notably, AR-positive luminal A male breast cancer had improved overall survival over female breast cancer at 5 (P = 0.01, HR = 0.39, 95% CI = 0.26-0.87) but not 10 years (P = 0.29, HR = 0.75, 95% CI = 0.46-1.26) and both 5 (P = 0.04, HR = 0.37, 95% CI = 0.07-0.97) and 10 years (P = 0.04, HR = 0.43, 95% CI = 0.12-0.97) in the unselected group. Hierarchical clustering revealed common clusters between genders including total PR-PRA-PRB and ERβ1/2 clusters. A striking feature was the occurrence of ERα on distinct clusters between genders. In female breast cancer, ERα clustered with PR and its isoforms; in male breast cancer, ERα clustered with ERβ isoforms and AR. Our data supports the hypothesis that breast cancer is biologically different in males and females suggesting implications for clinical management. With the incidence of male breast cancer increasing this provides impetus for further study.

Original languageEnglish
Pages (from-to)949-958
Number of pages10
JournalBreast Cancer Research and Treatment
Volume133
Issue number3
DOIs
Publication statusPublished - Jun 2012

Keywords

  • Androgen receptor
  • Hierarchical clustering
  • Hormone receptors
  • Male breast cancer

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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    Shaaban, A. M., Ball, G. R., Brannan, R. A., Cserni, G., Di Benedetto, A., Dent, J., Fulford, L., Honarpisheh, H., Jordan, L., Jones, J. L., Kanthan, R., Maraqa, L., Litwiniuk, M., Mottolese, M., Pollock, S., Provenzano, E., Quinlan, P. R., Reall, G., Shousha, S., ... Speirs, V. (2012). A comparative biomarker study of 514 matched cases of male and female breast cancer reveals gender-specific biological differences. Breast Cancer Research and Treatment, 133(3), 949-958. https://doi.org/10.1007/s10549-011-1856-9