A combined artificial chromosome-stem cell therapy method in a model experiment aimed at the treatment of Krabbe's disease in the Twitcher mouse

R. L. Katona, I. Sinkó, G. Holló, K. Székely Szucs, T. Praznovszky, J. Kereso, E. Csonka, K. Fodor, I. Cserpán, B. Szakál, P. Blazsó, A. Udvardy, G. Hadlaczky

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Mammalian artificial chromosomes (MACs) are safe, stable, non-integrating genetic vectors with almost unlimited therapeutic transgene-carrying capacity. The combination of MAC and stem cell technologies offers a new strategy for stem cell-based therapy, the efficacy of which was confirmed and validated by using a mouse model of a devastating monogenic disease, galactocerebrosidase deficiency (Krabbe's disease). Therapeutic MACs were generated by sequence-specific loading of galactocerebrosidase transgenes into a platform MAC, and stable, pluripotent mouse embryonic stem cell lines were established with these chromosomes. The transgenic stem cells were thoroughly characterized and used to produce chimeric mice on the mutant genetic background. The lifespan of these chimeras was increased twofold, verifying the feasibility of the development of MAC-stem cell systems for the delivery of therapeutic genes in stem cells to treat genetic diseases and cancers, and to produce cell types for cell replacement therapies.

Original languageEnglish
Pages (from-to)3830-3838
Number of pages9
JournalCellular and Molecular Life Sciences
Volume65
Issue number23
DOIs
Publication statusPublished - Nov 1 2008

Keywords

  • Gene therapy
  • Krabbe's disease
  • Mammalian artificial chromosome
  • Stem cell
  • Transgenic mouse

ASJC Scopus subject areas

  • Molecular Medicine
  • Molecular Biology
  • Pharmacology
  • Cellular and Molecular Neuroscience
  • Cell Biology

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  • Cite this

    Katona, R. L., Sinkó, I., Holló, G., Szucs, K. S., Praznovszky, T., Kereso, J., Csonka, E., Fodor, K., Cserpán, I., Szakál, B., Blazsó, P., Udvardy, A., & Hadlaczky, G. (2008). A combined artificial chromosome-stem cell therapy method in a model experiment aimed at the treatment of Krabbe's disease in the Twitcher mouse. Cellular and Molecular Life Sciences, 65(23), 3830-3838. https://doi.org/10.1007/s00018-008-8442-2