A color in situ hybridization method with improved sensitivity for the detection of low-abundance mRNAs

Beatrix Kovacs, Karoly Gulya

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

We have modified a nonradioactive (color) in situ hybridization method for the detection of mRNA populations, transcribed from the calmodulin (CaM) I gene, that exist in low abundances in tissues such as the white matter of the rat spinal cord. Our results indicate that increasing the pH of the hybridization solution from neutral to slightly alkaline (pH 8.0-8.5) drastically improves the detectable signal intensity of the digoxigenin-labeled CaM I gene-specific riboprobe while providing high spatial resolution. This method could be useful for the detection of other mRNA populations present in cells in low concentrations or in tissues where probe penetration might be impaired (e.g. a high lipid content).

Original languageEnglish
Pages (from-to)75-77
Number of pages3
JournalActa Biologica Szegediensis
Volume45
Issue number1-4
Publication statusPublished - Dec 1 2001

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Keywords

  • Alkaline pH
  • Calmodulin
  • Digoxigenin
  • Gene expression
  • In situ hybridization
  • Rat
  • Spinal cord
  • White matter
  • mRNA

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

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