A chemocentric approach to the identification of cancer targets.

Beáta Flachner, Zsolt Lörincz, Angelo Carotti, Orazio Nicolotti, Praveena Kuchipudi, Nikita Remez, Ferran Sanz, József Tóvári, Miklós J. Szabó, Béla Bertók, Sándor Cseh, Jordi Mestres, György Dormán

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12 Citations (Scopus)

Abstract

A novel chemocentric approach to identifying cancer-relevant targets is introduced. Starting with a large chemical collection, the strategy uses the list of small molecule hits arising from a differential cytotoxicity screening on tumor HCT116 and normal MRC-5 cell lines to identify proteins associated with cancer emerging from a differential virtual target profiling of the most selective compounds detected in both cell lines. It is shown that this smart combination of differential in vitro and in silico screenings (DIVISS) is capable of detecting a list of proteins that are already well accepted cancer drug targets, while complementing it with additional proteins that, targeted selectively or in combination with others, could lead to synergistic benefits for cancer therapeutics. The complete list of 115 proteins identified as being hit uniquely by compounds showing selective antiproliferative effects for tumor cell lines is provided.

Original languageEnglish
Pages (from-to)e35582
JournalPloS one
Volume7
Issue number4
DOIs
Publication statusPublished - 2012

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ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

Cite this

Flachner, B., Lörincz, Z., Carotti, A., Nicolotti, O., Kuchipudi, P., Remez, N., Sanz, F., Tóvári, J., Szabó, M. J., Bertók, B., Cseh, S., Mestres, J., & Dormán, G. (2012). A chemocentric approach to the identification of cancer targets. PloS one, 7(4), e35582. https://doi.org/10.1371/journal.pone.0035582