A cell-free system from Rhizobium meliloti to study the specific expression of nodulation genes

I. Dusha, J. Schroder, P. Putnoky, Z. Bánfalvi, A. Kondorosi

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

An in vitro transcription-translation system was developed using cell-free extracts from the symbiotic nitrogen-fixing bacterium Rhizobium meliloti strain 41. Conditions for preparation of the 30 000 x g supernatant extract and for measurement of protein-synthesizing activity were determined and compared to the activity of an Escherichia coli cell-free system. Genes expressed in the free-living or in the symbiotic state were studied. The product of a recA-like gene (41-kDa protein) was synthesized both in R. meliloti and E. coli extracts, although less efficiently in the heterologous system. In agreement with earlier results obtained in E. coli minicells, three proteins (44, 28.5 and 23 kDa) were synthesized from a cloned 3.3 x 103-base DNA region carrying genes for nodulation (nod). However, differences in the transcription-translation of nod and host specificity (hsn) genes were observed when protein expression was compared in R. meliloti and E. coli cell-free extracts, and the possible explanations of these findings are discussed.

Original languageEnglish
Pages (from-to)69-75
Number of pages7
JournalEuropean Journal of Biochemistry
Volume160
Issue number1
DOIs
Publication statusPublished - 1986

Fingerprint

Sinorhizobium meliloti
Cell-Free System
Escherichia coli
Genes
Gene Expression
Transcription
Cell Extracts
Proteins
Host Specificity
Bacteria
Nitrogen
DNA

ASJC Scopus subject areas

  • Biochemistry

Cite this

A cell-free system from Rhizobium meliloti to study the specific expression of nodulation genes. / Dusha, I.; Schroder, J.; Putnoky, P.; Bánfalvi, Z.; Kondorosi, A.

In: European Journal of Biochemistry, Vol. 160, No. 1, 1986, p. 69-75.

Research output: Contribution to journalArticle

Dusha, I. ; Schroder, J. ; Putnoky, P. ; Bánfalvi, Z. ; Kondorosi, A. / A cell-free system from Rhizobium meliloti to study the specific expression of nodulation genes. In: European Journal of Biochemistry. 1986 ; Vol. 160, No. 1. pp. 69-75.
@article{948ef2b0d15043169d4535d5f11a435e,
title = "A cell-free system from Rhizobium meliloti to study the specific expression of nodulation genes",
abstract = "An in vitro transcription-translation system was developed using cell-free extracts from the symbiotic nitrogen-fixing bacterium Rhizobium meliloti strain 41. Conditions for preparation of the 30 000 x g supernatant extract and for measurement of protein-synthesizing activity were determined and compared to the activity of an Escherichia coli cell-free system. Genes expressed in the free-living or in the symbiotic state were studied. The product of a recA-like gene (41-kDa protein) was synthesized both in R. meliloti and E. coli extracts, although less efficiently in the heterologous system. In agreement with earlier results obtained in E. coli minicells, three proteins (44, 28.5 and 23 kDa) were synthesized from a cloned 3.3 x 103-base DNA region carrying genes for nodulation (nod). However, differences in the transcription-translation of nod and host specificity (hsn) genes were observed when protein expression was compared in R. meliloti and E. coli cell-free extracts, and the possible explanations of these findings are discussed.",
author = "I. Dusha and J. Schroder and P. Putnoky and Z. B{\'a}nfalvi and A. Kondorosi",
year = "1986",
doi = "10.1111/j.1432-1033.1986.tb09941.x",
language = "English",
volume = "160",
pages = "69--75",
journal = "FEBS Journal",
issn = "1742-464X",
publisher = "Wiley-Blackwell",
number = "1",

}

TY - JOUR

T1 - A cell-free system from Rhizobium meliloti to study the specific expression of nodulation genes

AU - Dusha, I.

AU - Schroder, J.

AU - Putnoky, P.

AU - Bánfalvi, Z.

AU - Kondorosi, A.

PY - 1986

Y1 - 1986

N2 - An in vitro transcription-translation system was developed using cell-free extracts from the symbiotic nitrogen-fixing bacterium Rhizobium meliloti strain 41. Conditions for preparation of the 30 000 x g supernatant extract and for measurement of protein-synthesizing activity were determined and compared to the activity of an Escherichia coli cell-free system. Genes expressed in the free-living or in the symbiotic state were studied. The product of a recA-like gene (41-kDa protein) was synthesized both in R. meliloti and E. coli extracts, although less efficiently in the heterologous system. In agreement with earlier results obtained in E. coli minicells, three proteins (44, 28.5 and 23 kDa) were synthesized from a cloned 3.3 x 103-base DNA region carrying genes for nodulation (nod). However, differences in the transcription-translation of nod and host specificity (hsn) genes were observed when protein expression was compared in R. meliloti and E. coli cell-free extracts, and the possible explanations of these findings are discussed.

AB - An in vitro transcription-translation system was developed using cell-free extracts from the symbiotic nitrogen-fixing bacterium Rhizobium meliloti strain 41. Conditions for preparation of the 30 000 x g supernatant extract and for measurement of protein-synthesizing activity were determined and compared to the activity of an Escherichia coli cell-free system. Genes expressed in the free-living or in the symbiotic state were studied. The product of a recA-like gene (41-kDa protein) was synthesized both in R. meliloti and E. coli extracts, although less efficiently in the heterologous system. In agreement with earlier results obtained in E. coli minicells, three proteins (44, 28.5 and 23 kDa) were synthesized from a cloned 3.3 x 103-base DNA region carrying genes for nodulation (nod). However, differences in the transcription-translation of nod and host specificity (hsn) genes were observed when protein expression was compared in R. meliloti and E. coli cell-free extracts, and the possible explanations of these findings are discussed.

UR - http://www.scopus.com/inward/record.url?scp=0023008951&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023008951&partnerID=8YFLogxK

U2 - 10.1111/j.1432-1033.1986.tb09941.x

DO - 10.1111/j.1432-1033.1986.tb09941.x

M3 - Article

C2 - 3533532

AN - SCOPUS:0023008951

VL - 160

SP - 69

EP - 75

JO - FEBS Journal

JF - FEBS Journal

SN - 1742-464X

IS - 1

ER -