A CCD-OMA device for the measurement of complete chlorophyll fluorescence emission spectra of leaves during the fluorescence induction kinetics

K. Szabó, H. K. Lichtenthaler, L. Kocsányi, P. Richter

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

A new device for the measurement of complete laser induced fluorescence emission spectra (maxima near 690 and 735 nm) of leaves during the induction of the chlorophyll fluorescence is described. In this the excitation light (cw He/Ne laser, 632.8 nm) is switched on by a fast electro-mechanical shutter which provides an opening time of 1 ms. The emitted fluorescence is imaged onto the entrance slit of a multichannel spectrograph through a red cut-off filter (> 645 nm). A charge coupled device (CCD) sensor with 2048 elements simultaneously detects the complete chlorophyll fluorescence emission spectrum in the 650-800 nm wavelength range. Scanning is accomplished electronically and the integration time for a complete fluorescence emission spectrum can be selected from 10 ms up to 260 ms. Shutter, detector system and data acquisition are controlled by an IBM-PC/AT compatible computer. A maximum of 32 spectra can be measured at selected times during the fluorescence induction kinetics with the shortest time resolution of 10 ms. The instrument permits the determination of various fluorescence parameters:a) the rise-time of the fluorescence to the maximum level fm, b) the changes in the shape of the fluorescence emission spectra during the induction kinetics, c) the induction kinetics in the fluorescence ratio F690/F735 as well as d) the fluorescence decrease ratio Rfd at any wavelength between 650 to 800 nm. These fluorescence parameters provide information about the functioning of photosynthesis. The ratio F690/F735 allows the non-destructive determination of the chlorophyll content of leaves. The application of this instrument in ecophysiological research and stress physiology of plants is outlined.

Original languageEnglish
Pages (from-to)153-160
Number of pages8
JournalRadiation and Environmental Biophysics
Volume31
Issue number2
DOIs
Publication statusPublished - Jun 1992

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chlorophylls
Chlorophyll
Charge coupled devices
leaves
charge coupled devices
induction
chlorophyll
emission spectra
fluorescence
Fluorescence
kinetics
Equipment and Supplies
Kinetics
shutters
stress (physiology)
lasers
wavelengths
Lasers
IBM personal computers
wavelength

ASJC Scopus subject areas

  • Radiation
  • Physics and Astronomy (miscellaneous)
  • Radiology Nuclear Medicine and imaging
  • Environmental Chemistry
  • Environmental Science (miscellaneous)
  • Environmental Science(all)
  • Biophysics
  • Agricultural and Biological Sciences (miscellaneous)

Cite this

A CCD-OMA device for the measurement of complete chlorophyll fluorescence emission spectra of leaves during the fluorescence induction kinetics. / Szabó, K.; Lichtenthaler, H. K.; Kocsányi, L.; Richter, P.

In: Radiation and Environmental Biophysics, Vol. 31, No. 2, 06.1992, p. 153-160.

Research output: Contribution to journalArticle

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