A brain specific water soluble antigen in homogenates of cat cerebral cortex

A. Orosz, A. Falus, E. Madarász, J. Gergely, G. Adám

Research output: Contribution to journalArticle

Abstract

Rabbits were immunized with the phospholipid free supernatant of an aqueous homogenate of cat cerebral cortex. From the antiserum obtained, the antibodies produced against the cat serum proteins, and the common antigens of other organs, were eliminated. The IgG (gamma G immunoglobulin) fraction was isolated from the completely absorbed antiserum. The antibodies prepared gave immunoprecipitation with a water soluble antigen having electrophoretic mobility similar to the β1 to β2 globulins found in human serum. Further purification of the antigen was carried out by means of anion exchange chromatography and analytical polyacrylamide gel electrophoresis.

Original languageEnglish
Pages (from-to)319-326
Number of pages8
JournalActa Biochimica et Biophysica Academiae Scientiarum Hungaricae
Volume9
Issue number4
Publication statusPublished - 1974

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Cerebral Cortex
Cats
Antigens
Water
Immune Sera
Brain
Immunoglobulin G
Antibodies
Globulins
Immunoprecipitation
Anions
Chromatography
Blood Proteins
Polyacrylamide Gel Electrophoresis
Phospholipids
Rabbits
Serum

ASJC Scopus subject areas

  • Medicine(all)

Cite this

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T1 - A brain specific water soluble antigen in homogenates of cat cerebral cortex

AU - Orosz, A.

AU - Falus, A.

AU - Madarász, E.

AU - Gergely, J.

AU - Adám, G.

PY - 1974

Y1 - 1974

N2 - Rabbits were immunized with the phospholipid free supernatant of an aqueous homogenate of cat cerebral cortex. From the antiserum obtained, the antibodies produced against the cat serum proteins, and the common antigens of other organs, were eliminated. The IgG (gamma G immunoglobulin) fraction was isolated from the completely absorbed antiserum. The antibodies prepared gave immunoprecipitation with a water soluble antigen having electrophoretic mobility similar to the β1 to β2 globulins found in human serum. Further purification of the antigen was carried out by means of anion exchange chromatography and analytical polyacrylamide gel electrophoresis.

AB - Rabbits were immunized with the phospholipid free supernatant of an aqueous homogenate of cat cerebral cortex. From the antiserum obtained, the antibodies produced against the cat serum proteins, and the common antigens of other organs, were eliminated. The IgG (gamma G immunoglobulin) fraction was isolated from the completely absorbed antiserum. The antibodies prepared gave immunoprecipitation with a water soluble antigen having electrophoretic mobility similar to the β1 to β2 globulins found in human serum. Further purification of the antigen was carried out by means of anion exchange chromatography and analytical polyacrylamide gel electrophoresis.

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