A 5-HT1A-like receptor is involved in the regulation of the embryonic rotation of Lymnaea stagnalis L.

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Abstract

Cilia driven rotation of the pond snail Lymnaea stagnalis embryos is regulated by serotonin (5-HT). In the present study, physiological and biochemical assays were used to identify the 5-HT receptor type involved in rotation. The 5-HTergic agonists applied stimulated the rotation by 180-400% and their rank order potency was as follows: LSD > 5-HT > 8-OH-DPAT > WB4101 ≫ 5-CT. The applied antagonists, spiperone, propranalol and mianserin inhibited the 5-HT or 8-OH-DPAT stimulated rotation of the embryos by 50-70%. 3H-5-HT was bound specifically to the washed pellet of the embryo homogenates. The specific binding of 3H-5-HT was saturable and showed a single, high affinity binding site with Kd 7.36 nM and Bmax 221 fmol/mg pellet values. This is the first report demonstrating the high affinity binding of 3H-5-HT to the native receptor in molluscs. All of the pharmacons that stimulated the rotation or inhibited the 5-HT or 8-OH-DPAT evoked stimulation displaced effectively the binding of 3H-5-HT. 5-HT resulted in the inhibition of forskolin stimulated cAMP accumulation, showing that 5-HT is negatively coupled to adenylate cyclase. Our results suggest that in the 5-HTergic regulation of the embryonic rotation in L. stagnalis a 5-HT1A-like receptor of the vertebrate type is involved.

Original languageEnglish
Pages (from-to)57-61
Number of pages5
JournalComparative Biochemistry and Physiology - C Toxicology and Pharmacology
Volume152
Issue number1
DOIs
Publication statusPublished - Jun 2010

Fingerprint

Lymnaea
Receptor, Serotonin, 5-HT1A
Serotonin
8-Hydroxy-2-(di-n-propylamino)tetralin
Embryonic Structures
Molluscs
Mianserin
Spiperone
Lysergic Acid Diethylamide
Cilia
Mollusca
Serotonin Receptors
Snails
Ponds
Colforsin
Adenylyl Cyclases
Vertebrates
Assays
Binding Sites

Keywords

  • 5-HT
  • Embryo
  • Lymnaea
  • Receptor

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Physiology
  • Health, Toxicology and Mutagenesis
  • Toxicology

Cite this

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title = "A 5-HT1A-like receptor is involved in the regulation of the embryonic rotation of Lymnaea stagnalis L.",
abstract = "Cilia driven rotation of the pond snail Lymnaea stagnalis embryos is regulated by serotonin (5-HT). In the present study, physiological and biochemical assays were used to identify the 5-HT receptor type involved in rotation. The 5-HTergic agonists applied stimulated the rotation by 180-400{\%} and their rank order potency was as follows: LSD > 5-HT > 8-OH-DPAT > WB4101 ≫ 5-CT. The applied antagonists, spiperone, propranalol and mianserin inhibited the 5-HT or 8-OH-DPAT stimulated rotation of the embryos by 50-70{\%}. 3H-5-HT was bound specifically to the washed pellet of the embryo homogenates. The specific binding of 3H-5-HT was saturable and showed a single, high affinity binding site with Kd 7.36 nM and Bmax 221 fmol/mg pellet values. This is the first report demonstrating the high affinity binding of 3H-5-HT to the native receptor in molluscs. All of the pharmacons that stimulated the rotation or inhibited the 5-HT or 8-OH-DPAT evoked stimulation displaced effectively the binding of 3H-5-HT. 5-HT resulted in the inhibition of forskolin stimulated cAMP accumulation, showing that 5-HT is negatively coupled to adenylate cyclase. Our results suggest that in the 5-HTergic regulation of the embryonic rotation in L. stagnalis a 5-HT1A-like receptor of the vertebrate type is involved.",
keywords = "5-HT, Embryo, Lymnaea, Receptor",
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AU - Hiripi, L.

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N2 - Cilia driven rotation of the pond snail Lymnaea stagnalis embryos is regulated by serotonin (5-HT). In the present study, physiological and biochemical assays were used to identify the 5-HT receptor type involved in rotation. The 5-HTergic agonists applied stimulated the rotation by 180-400% and their rank order potency was as follows: LSD > 5-HT > 8-OH-DPAT > WB4101 ≫ 5-CT. The applied antagonists, spiperone, propranalol and mianserin inhibited the 5-HT or 8-OH-DPAT stimulated rotation of the embryos by 50-70%. 3H-5-HT was bound specifically to the washed pellet of the embryo homogenates. The specific binding of 3H-5-HT was saturable and showed a single, high affinity binding site with Kd 7.36 nM and Bmax 221 fmol/mg pellet values. This is the first report demonstrating the high affinity binding of 3H-5-HT to the native receptor in molluscs. All of the pharmacons that stimulated the rotation or inhibited the 5-HT or 8-OH-DPAT evoked stimulation displaced effectively the binding of 3H-5-HT. 5-HT resulted in the inhibition of forskolin stimulated cAMP accumulation, showing that 5-HT is negatively coupled to adenylate cyclase. Our results suggest that in the 5-HTergic regulation of the embryonic rotation in L. stagnalis a 5-HT1A-like receptor of the vertebrate type is involved.

AB - Cilia driven rotation of the pond snail Lymnaea stagnalis embryos is regulated by serotonin (5-HT). In the present study, physiological and biochemical assays were used to identify the 5-HT receptor type involved in rotation. The 5-HTergic agonists applied stimulated the rotation by 180-400% and their rank order potency was as follows: LSD > 5-HT > 8-OH-DPAT > WB4101 ≫ 5-CT. The applied antagonists, spiperone, propranalol and mianserin inhibited the 5-HT or 8-OH-DPAT stimulated rotation of the embryos by 50-70%. 3H-5-HT was bound specifically to the washed pellet of the embryo homogenates. The specific binding of 3H-5-HT was saturable and showed a single, high affinity binding site with Kd 7.36 nM and Bmax 221 fmol/mg pellet values. This is the first report demonstrating the high affinity binding of 3H-5-HT to the native receptor in molluscs. All of the pharmacons that stimulated the rotation or inhibited the 5-HT or 8-OH-DPAT evoked stimulation displaced effectively the binding of 3H-5-HT. 5-HT resulted in the inhibition of forskolin stimulated cAMP accumulation, showing that 5-HT is negatively coupled to adenylate cyclase. Our results suggest that in the 5-HTergic regulation of the embryonic rotation in L. stagnalis a 5-HT1A-like receptor of the vertebrate type is involved.

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