A 268 bp upstream sequence mediates the circadian clock-regulated transcription of the wheat Cab-1 gene in transgenic plants

Erzsebet Fejes, Aniko Pay, Ivan Kanevsky, M. Széll, E. Ádám, Steven Kay, Ferenc Nagy

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

We previously reported that the expression of the wheat Cab-1 gene is regulated by an endogenous circadian rhythm and by the photoreceptor phytochrome both in wheat and in transgenic tobacco plants. To define regulatory elements necessary for the circadian rhythm-regulated Cab-1 gene expression, we now analysed the fluctuation of steady-state mRNA levels in a series of 5′ deletion mutants in transgenic tobacco plants. We found that the expression of a deletion mutant containing 211 bp upstream sequence still exhibited circadian rhythm. Furthermore we show that an enhancer-like sequence of the Cab-1 promoter (from -357 to -90) can endow a chimaeric gene consisting of a truncated 35S promoter (from -90 to +8) and the bacterial β-glucuronidase (GUS) gene with circadian clock-regulated gene expression. Finally we demonstrate by nuclear run-off experiments that the transcription rates of the Cab genes in wheat oscillate in a rhythmic manner, with a periodicity of approximately 24 hours. Consistent with our previous findings these results (i) indicate that the expression of the wheat Cab-1 gene is regulated mainly at the transcription level and (ii) identify a short promoter region between -211 and -90 that is responsible for the circadian clock-regulated gene expression.

Original languageEnglish
Pages (from-to)921-932
Number of pages12
JournalPlant Molecular Biology
Volume15
Issue number6
DOIs
Publication statusPublished - Dec 1990

Fingerprint

Circadian Clocks
Genetically Modified Plants
Transcription
circadian rhythm
Triticum
Clocks
transgenic plants
transcription (genetics)
Circadian Rhythm
Genes
wheat
Gene expression
Gene Expression
Tobacco
promoter regions
genes
gene expression
Phytochrome
Bacterial Genes
tobacco

Keywords

  • circadian rhythm
  • enhancer
  • transcription
  • transgenic plants

ASJC Scopus subject areas

  • Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

A 268 bp upstream sequence mediates the circadian clock-regulated transcription of the wheat Cab-1 gene in transgenic plants. / Fejes, Erzsebet; Pay, Aniko; Kanevsky, Ivan; Széll, M.; Ádám, E.; Kay, Steven; Nagy, Ferenc.

In: Plant Molecular Biology, Vol. 15, No. 6, 12.1990, p. 921-932.

Research output: Contribution to journalArticle

Fejes, Erzsebet ; Pay, Aniko ; Kanevsky, Ivan ; Széll, M. ; Ádám, E. ; Kay, Steven ; Nagy, Ferenc. / A 268 bp upstream sequence mediates the circadian clock-regulated transcription of the wheat Cab-1 gene in transgenic plants. In: Plant Molecular Biology. 1990 ; Vol. 15, No. 6. pp. 921-932.
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