9’-Z-epoxycarotenoid cleavage enzyme activity from the Citrus cell-free system for ABA biosynthesis

M. Kalala, A. K. Cowan, P. Molnár, G. Tóth

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The rate limiting step in abscisic acid (ABA) biosynthesis is believed to be the dioxygenase-mediated cleavage of 9-Z-epoxy carotenoids. Isolation of an enzyme that cleaves 9'-Z-neoxanthin to xanthoxal (XAN) from the Citrus cell-free system for ABA biosynthesis is described. The enzyme was precipitated using 45% ammonium sulphate and resolved from lipoxygenase (LOX) by low pressure liquid chromatography on a column of phenyl sepharose. Activity of the enzyme was enhanced from 5.32 to 15.96μg XAN mg-1 protein confirming that 9'-Z-neoxanthin is readily converted to XAN in the Citrus cell-free system.

Original languageEnglish
Pages (from-to)376-377
Number of pages2
JournalSouth African Journal of Botany
Volume67
Issue number2
DOIs
Publication statusPublished - Jan 1 2001

ASJC Scopus subject areas

  • Plant Science

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