7S RNA, containing 5S ribosomal RNA and the termination stem, is a specific substrate for the two RNA processing enzymes RNase III and RNase E

J. Szeberényi, Monoj K. Roy, Hemant C. Vaidya, David Apirion

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The 7S RNA, a precursor of 5S rRNA that contains 5S rRNA and the termination stem and loop, is a substrate for RNase E and is also a substrate for RNase III. The cleavage by RNase III is in the stem, 11 nucleotides downstream from the 3′ end of the mature 5S rRNA and 8 nucleotides downstream from the RNase E cleavage site. Near the cleaved nucleotides there are three base pairs that appear in the same relative positions in most known RNase III cleavage sites. The large product of the RNase III cleavage reaction, which is a 5S rRNA that contains 11 extra nucleotides at the 3′ end, is a substrate for RNase E. This suggests that the information for the 3′-end cleavage by RNase E resides mainly in the 5S rRNA itself. Using rnc rne strains, carrying the plasmid that leads to the accumulation of 7S RNA, we showed that the 7S RNA does not result from an RNase III cleavage but is apparently a proper transcription termination product.

Original languageEnglish
Pages (from-to)2952-2957
Number of pages6
JournalBiochemistry
Volume23
Issue number13
Publication statusPublished - 1984

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5S Ribosomal RNA
Ribonuclease III
RNA
Nucleotides
Substrates
Enzymes
Processing
RNA Precursors
Transcription
Base Pairing
Plasmids
7SL RNA
ribonuclease E

ASJC Scopus subject areas

  • Biochemistry

Cite this

7S RNA, containing 5S ribosomal RNA and the termination stem, is a specific substrate for the two RNA processing enzymes RNase III and RNase E. / Szeberényi, J.; Roy, Monoj K.; Vaidya, Hemant C.; Apirion, David.

In: Biochemistry, Vol. 23, No. 13, 1984, p. 2952-2957.

Research output: Contribution to journalArticle

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