7 S RNA: A single site substrate for the RNA processing enzyme ribonuclease E of Escherichia coli

József Szeberényi, Monoj K. Roy, David Apirion

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

7 S RNA accumulates at non-permissive temperatures in an RNAase E strain containing the recombinant plasmid pJR3Δ which carries a single 5 S rRNA gene and expression sequences. 7 S RNA is a processing intermediate that contains the complete sequence of 5 S rRNA as well as a stem-and-loop structure encoded by the terminator of rrnD. 7 S RNA can be processed in vitro by RNAase E. Structural analysis of the products (5 S rRNA and the stem) of in vitro processing of 7 S RNA revealed that the cleavage site of RNAase E in 7 S RNA is 3 nucleotides downstream from the 3′ end of the mature 5 S rRNA. The cleavage generates 3′-hydroxyl and 5′-phosphate termini.

Original languageEnglish
Pages (from-to)282-290
Number of pages9
JournalBBA - Gene Structure and Expression
Volume740
Issue number3
DOIs
Publication statusPublished - Aug 2 1983

Keywords

  • (E. coli)
  • 5 S rRNA
  • 7 S RNA intermediate
  • RNA processing
  • Ribonuclease E

ASJC Scopus subject areas

  • Structural Biology
  • Biophysics
  • Biochemistry
  • Genetics

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