This chapter concentrates primarily on techniques involved in studies of the heat-shock phenomenon in cyanobacteria. For convenience of discussion only the heat shock of Synechococcus Strain PCC 6301 is described, but these methods have direct applicability in studies directed toward the stress responses of other cyanobacterial strains as well. The techniques are simple and easy to execute. The specific pattern of protein synthesis can be monitored readily by use of two-dimensional polyacrylamide gel electrophoresis. Growing cyanobacteria are strongly influenced by their nutritional, chemical, and physical environments. Of those factors, temperature plays a critical role: cyanobacteria exposed to temperatures higher than those for normal growth respond by altering patterns of growth and protein synthesis. One of the most emphasized aspects of light-dependent cyanobacterial gene expression is its pleiotropic nature; hence, a specific manipulation of cyanobacterial genes or regulons could simplify attempts to understand the regulation of gene expression in these organisms. Accordingly, studies on heat shock and other stress responses are of interest not only in their own right but from the viewpoint of light-dependent and regulated cyanobacterial gene expression as well.
ASJC Scopus subject areas
- Molecular Biology