5-HT1B and 5-HT1D receptors in the human trigeminal ganglion: Co-localization with calcitonin gene-related peptide, substance P and nitric oxide synthase

Mingyan Hou, Martin Kanje, Jenny Longmore, Janos Tajti, Rolf Uddman, Lars Edvinsson

Research output: Contribution to journalArticle

115 Citations (Scopus)

Abstract

5-Hydroxytryptamine (5-HT) is implicated in migraine and agonist directed aganist 5-HT1B and 5-HT1D receptors are commonly used as effective therapies. The antimigraine mechanisms involve the inhibition of intracranial sensory neuropeptide release. In order to determine which 5-HT1 receptor subtypes are involved we have by immunocytochemistry examined the distribution of 5-HT1B and 5-HT1D receptors in the human trigeminal ganglia, and addressed which of them colocalize with calcitonin gene-related peptide (CGRP), substance P (SP) or nitric oxide synthase (NOS). We detected that 5-HT1D receptor immunoreactivity (i.r.) was predominantly expressed in medium-sized cells (86% of positive cells, 30-60 μm). About 9% of the 5-HT1D receptor i.r. cells were large in size (>60 μm) and 5% were small in size (<30 μm). In a similar pattern, 5-HT1B receptor i.r. was mainly expressed in medium-sized cells (81% in 30-60 μm, 15% in >60 μm and 4% in <30 μm). Double immunostaining was used to determine whether the 5-HT1B or 5-HT1D receptor immunoreactive cells co-localized with either CGRP, SP or NOS. Thus, 89% of the CGRP i.r. cells expressed 5-HT1D receptor i.r. and 65% of the CGRP positive cells were 5-HT1B receptor positive. Most of the 5-HT1D (95%) and the 5-HT1B (94%) receptor i.r. cells showed SP immunostaining and 83% of 5-HT1D receptor and 86% of 5-HT1B receptor i.r. cells contained NOS. In conclusion, both 5-HT1B and 5-HT1D receptors are expressed in the human trigeminal ganglion and they are mainly localized in medium-sized cells and they seem to colocalize with CGRP, SP and NOS.

Original languageEnglish
Pages (from-to)112-120
Number of pages9
JournalBrain research
Volume909
Issue number1-2
DOIs
Publication statusPublished - Aug 3 2001

Keywords

  • 5-HT and 5-HT receptors
  • Calcitonin gene-related peptide
  • Nitric oxide synthase and immunocytochemistry
  • Substance P

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

Fingerprint Dive into the research topics of '5-HT<sub>1B</sub> and 5-HT<sub>1D</sub> receptors in the human trigeminal ganglion: Co-localization with calcitonin gene-related peptide, substance P and nitric oxide synthase'. Together they form a unique fingerprint.

  • Cite this