4-Thio-uridylate (UD29) interferes with the function of protein -SH and inhibits HIV replication in vitro

Zoltán Beck, Andrea Kis, Erika Berényi, Péter Kovács, L. Fésüs, J. Aradi

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

In this short communication, it is shown that 4-thio-uridylate (s4UMP, designated as UD29) inhibits glyceraldehyde 3-phosphate dehydrogenase (GAPDH), suggesting that the enol-form of the thiolated nucleotide may interfere with the function of the essential -SH group in the active center of the enzyme. Since HIV entry requires thiol/disulfide exchange processes, this activity prompted us to study the anti-HIV activity of the nucleotide. Indeed, UD29 inhibited the replication of HIV-1IIIB in the MT-4 cell line and HIV-1Ada-M in peripheral blood mononuclear cells (PBMC). Furthermore, UD29 was not toxic in PBMCs in vitro or in mice when the compound was administered intravenously.

Original languageEnglish
Pages (from-to)343-347
Number of pages5
JournalPharmacological Reports
Volume61
Issue number2
Publication statusPublished - 2009

Fingerprint

HIV
Proteins
Nucleotides
Glyceraldehyde-3-Phosphate Dehydrogenases
Poisons
Sulfhydryl Compounds
Disulfides
Blood Cells
Cell Line
In Vitro Techniques
Enzymes

Keywords

  • Anti-HIV
  • HIV-entry
  • Pyrimidine nucleotide
  • Thiol/disulfide redox process

ASJC Scopus subject areas

  • Pharmacology

Cite this

4-Thio-uridylate (UD29) interferes with the function of protein -SH and inhibits HIV replication in vitro. / Beck, Zoltán; Kis, Andrea; Berényi, Erika; Kovács, Péter; Fésüs, L.; Aradi, J.

In: Pharmacological Reports, Vol. 61, No. 2, 2009, p. 343-347.

Research output: Contribution to journalArticle

Beck, Zoltán ; Kis, Andrea ; Berényi, Erika ; Kovács, Péter ; Fésüs, L. ; Aradi, J. / 4-Thio-uridylate (UD29) interferes with the function of protein -SH and inhibits HIV replication in vitro. In: Pharmacological Reports. 2009 ; Vol. 61, No. 2. pp. 343-347.
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