The effect of serine protease inhibitor 4-(2-aminoethyl)benzenesulfonyl fluoride (AEBSF) was investigated on the prevention of tumor-necrosis-factor-α (TNF-α)-induced blood-brain barrier opening. TNF-α (10,000 IU) was injected intracarotidly to newborn pigs pretreated with 0, 2.4, 4.8, 9.6 and 19.2 mg/kg AEBSF (n=6 in each group). AEBSF dose-dependently inhibited the TNF-α-induced increase in the blood-brain barrier permeability for sodium fluorescein (MW=376) in all of the five brain regions examined, while only 19.2 mg/kg AEBSF could significantly (P<0.05) decrease the change in Evan's blue-albumin (MW=67,000) transport in two regions. In conclusion, AEBSF attenuates vasogenic brain edema formation. Copyright (C) 1999 Elsevier Science B.V.
- AEBSF [4-(2-aminoethyl)benzenesulfonyl] fluoride
- Blood-brain barrier
- Brain edema
- Serine protease inhibitor
- TNF-α (tumor necrosis factor-α)
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