2D FT-IR spectroscopy analysis of the pressure-induced changes in proteins

L. Smeller, K. Heremans

Research output: Contribution to journalConference article

50 Citations (Scopus)


In this paper, we apply for the first time two-dimensional (2D) correlation spectroscopy to analyze pressure-induced changes in proteins. We show that it is possible to distinguish between hydrogen-deuterium (H/D) exchange and conformational changes from the synchronous and the asynchronous spectra. From the sequence of the spectral changes it can be concluded that the initial partial unfolding of the secondary structure enhances the exchange, which is followed by further conformational changes. The exchange process is complete below ca. 0.4 GPa. The elastic distortions of the protein at higher pressures seem not to be accompanied by H/D exchange.

Original languageEnglish
Pages (from-to)375-378
Number of pages4
JournalVibrational Spectroscopy
Issue number2
Publication statusPublished - Jan 1 1999
EventProceedings of the 1998 3rd International Symposium on Advanced Infrared and Raman Spectroscopy, AIRS III - Vienna, Austria
Duration: Jul 5 1998Jul 9 1998


  • FT-IR spectroscopy
  • Proteins
  • Two-dimensional (2D) correlation spectroscopy

ASJC Scopus subject areas

  • Spectroscopy

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