This is the first report of sucessful embryo transfer in horses in Hungary. The work has been carried out at the Experimental Institute of the University of Veterinary Science in the autumn of 1994 and in the summer of 1995. Ovarian activity was followed by daily rectal examination and ultrasonography (ALOKA SSD-210 DX II, 5 MHz transducer, ALOKA Co., Japan) during the examination period. For the synchronisation of the donors and recipients PGF2α (Enzaprost 25 inj. A. U. V., Sanofi, France) was used at 10 mg dose rate. In the first part of the study (1994) 10 recoveries were performed on 8 mares on day 7 (day 0 = day of ovulation) of the cycle. Out of the 10 attempts 8 was evaluated. In one case systemic disease was present and in the other case the behaviour of the mare prevented the flushing. The 8 recoveries resulted in 6 embryos (6/8 = 75%) (4 early and 2 expanded blastocyst) and 5 was considered to be of transferable quality (5/8 = 62,5%) (Figure 1). In the second part of the study (1995) 2 mares were used as donor and 3 as recipient, respectively. 5 recoveries were performed and 2 embryos (early blastocyst of excellent to good quality) and 1 oocyte were collected. The two embryos were transferred to recipients ovulating 1 or 2 days after the donors with non surgical, transcervical technique. 7 days after the transfer an embryonic vesicle was found in both recipients but one of them underwent early embryonic death 2 days later. The other continued in normal pregnancy (Figure 2) and a living foal was born in the summer of 1996 (Figure 3).
|Number of pages||4|
|Journal||Magyar Allatorvosok Lapja|
|Publication status||Published - Dec 1 1996|
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