γ-Glutamyl transpeptidase (GgtA) of Aspergillus nidulans is not necessary for bulk degradation of glutathione

Zsolt Spitzmüller, Nak Jung Kwon, Melinda Szilágyi, Judit Keserű, Viktória Tóth, Jae Hyuk Yu, I. Pócsi, T. Emri

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Aspergillus nidulans exhibited high γ-glutamyl transpeptidase (γGT) activity in both carbon-starved and carbon-limited cultures. Glucose repressed, but casein peptone increased γGT production. Null mutation of creA did not influence γGT formation, but the functional meaB was necessary for the γGT induction. Deletion of the AN10444 gene (ggtA) completely eliminated the γGT activity, and the mRNA levels of ggtA showed strong correlation with the observed γGT activities. While ggtA does not contain a canonical signal sequence, the γGT activity was detectable both in the fermentation broth and in the hyphae. Deletion of the ggtA gene did not prevent the depletion of glutathione observed in carbon-starved and carbon-limited cultures. Addition of casein peptone to carbon-starved cultures lowered the formation of reactive species (RS). Deletion of ggtA could hinder this decrease and resulted in elevated RS formation. This effect of γGT on redox homeostasis may explain the reduced cleistothecia formation of ΔggtA strains in surface cultures.

Original languageEnglish
Pages (from-to)285-297
Number of pages13
JournalArchives of Microbiology
Volume197
Issue number2
DOIs
Publication statusPublished - 2014

Fingerprint

Aspergillus nidulans
gamma-Glutamyltransferase
Aspergillus
Glutathione
Degradation
Carbon
Gene Deletion
Genes
Hyphae
Protein Sorting Signals
Fermentation
Oxidation-Reduction
Homeostasis
Glucose
Messenger RNA
Mutation

Keywords

  • Aspergillus nidulans
  • Carbon limitation
  • Carbon starvation
  • Glutathione
  • MeaB
  • Sexual development
  • γ-Glutamyl transpeptidase

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Biochemistry
  • Genetics
  • Medicine(all)

Cite this

γ-Glutamyl transpeptidase (GgtA) of Aspergillus nidulans is not necessary for bulk degradation of glutathione. / Spitzmüller, Zsolt; Kwon, Nak Jung; Szilágyi, Melinda; Keserű, Judit; Tóth, Viktória; Yu, Jae Hyuk; Pócsi, I.; Emri, T.

In: Archives of Microbiology, Vol. 197, No. 2, 2014, p. 285-297.

Research output: Contribution to journalArticle

Spitzmüller, Zsolt ; Kwon, Nak Jung ; Szilágyi, Melinda ; Keserű, Judit ; Tóth, Viktória ; Yu, Jae Hyuk ; Pócsi, I. ; Emri, T. / γ-Glutamyl transpeptidase (GgtA) of Aspergillus nidulans is not necessary for bulk degradation of glutathione. In: Archives of Microbiology. 2014 ; Vol. 197, No. 2. pp. 285-297.
@article{a6e32a937d314ca7ac481c6020d8acd0,
title = "γ-Glutamyl transpeptidase (GgtA) of Aspergillus nidulans is not necessary for bulk degradation of glutathione",
abstract = "Aspergillus nidulans exhibited high γ-glutamyl transpeptidase (γGT) activity in both carbon-starved and carbon-limited cultures. Glucose repressed, but casein peptone increased γGT production. Null mutation of creA did not influence γGT formation, but the functional meaB was necessary for the γGT induction. Deletion of the AN10444 gene (ggtA) completely eliminated the γGT activity, and the mRNA levels of ggtA showed strong correlation with the observed γGT activities. While ggtA does not contain a canonical signal sequence, the γGT activity was detectable both in the fermentation broth and in the hyphae. Deletion of the ggtA gene did not prevent the depletion of glutathione observed in carbon-starved and carbon-limited cultures. Addition of casein peptone to carbon-starved cultures lowered the formation of reactive species (RS). Deletion of ggtA could hinder this decrease and resulted in elevated RS formation. This effect of γGT on redox homeostasis may explain the reduced cleistothecia formation of ΔggtA strains in surface cultures.",
keywords = "Aspergillus nidulans, Carbon limitation, Carbon starvation, Glutathione, MeaB, Sexual development, γ-Glutamyl transpeptidase",
author = "Zsolt Spitzm{\"u}ller and Kwon, {Nak Jung} and Melinda Szil{\'a}gyi and Judit Keserű and Vikt{\'o}ria T{\'o}th and Yu, {Jae Hyuk} and I. P{\'o}csi and T. Emri",
year = "2014",
doi = "10.1007/s00203-014-1057-0",
language = "English",
volume = "197",
pages = "285--297",
journal = "Archives of Microbiology",
issn = "0302-8933",
publisher = "Springer Verlag",
number = "2",

}

TY - JOUR

T1 - γ-Glutamyl transpeptidase (GgtA) of Aspergillus nidulans is not necessary for bulk degradation of glutathione

AU - Spitzmüller, Zsolt

AU - Kwon, Nak Jung

AU - Szilágyi, Melinda

AU - Keserű, Judit

AU - Tóth, Viktória

AU - Yu, Jae Hyuk

AU - Pócsi, I.

AU - Emri, T.

PY - 2014

Y1 - 2014

N2 - Aspergillus nidulans exhibited high γ-glutamyl transpeptidase (γGT) activity in both carbon-starved and carbon-limited cultures. Glucose repressed, but casein peptone increased γGT production. Null mutation of creA did not influence γGT formation, but the functional meaB was necessary for the γGT induction. Deletion of the AN10444 gene (ggtA) completely eliminated the γGT activity, and the mRNA levels of ggtA showed strong correlation with the observed γGT activities. While ggtA does not contain a canonical signal sequence, the γGT activity was detectable both in the fermentation broth and in the hyphae. Deletion of the ggtA gene did not prevent the depletion of glutathione observed in carbon-starved and carbon-limited cultures. Addition of casein peptone to carbon-starved cultures lowered the formation of reactive species (RS). Deletion of ggtA could hinder this decrease and resulted in elevated RS formation. This effect of γGT on redox homeostasis may explain the reduced cleistothecia formation of ΔggtA strains in surface cultures.

AB - Aspergillus nidulans exhibited high γ-glutamyl transpeptidase (γGT) activity in both carbon-starved and carbon-limited cultures. Glucose repressed, but casein peptone increased γGT production. Null mutation of creA did not influence γGT formation, but the functional meaB was necessary for the γGT induction. Deletion of the AN10444 gene (ggtA) completely eliminated the γGT activity, and the mRNA levels of ggtA showed strong correlation with the observed γGT activities. While ggtA does not contain a canonical signal sequence, the γGT activity was detectable both in the fermentation broth and in the hyphae. Deletion of the ggtA gene did not prevent the depletion of glutathione observed in carbon-starved and carbon-limited cultures. Addition of casein peptone to carbon-starved cultures lowered the formation of reactive species (RS). Deletion of ggtA could hinder this decrease and resulted in elevated RS formation. This effect of γGT on redox homeostasis may explain the reduced cleistothecia formation of ΔggtA strains in surface cultures.

KW - Aspergillus nidulans

KW - Carbon limitation

KW - Carbon starvation

KW - Glutathione

KW - MeaB

KW - Sexual development

KW - γ-Glutamyl transpeptidase

UR - http://www.scopus.com/inward/record.url?scp=84925460930&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84925460930&partnerID=8YFLogxK

U2 - 10.1007/s00203-014-1057-0

DO - 10.1007/s00203-014-1057-0

M3 - Article

C2 - 25519188

AN - SCOPUS:84925460930

VL - 197

SP - 285

EP - 297

JO - Archives of Microbiology

JF - Archives of Microbiology

SN - 0302-8933

IS - 2

ER -