γ/δ T lymphocytes in Mycobacterium tuberculosis infection

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Background - Data on the percentage of γ/δ T lymphocytes in the peripheral blood of patients infected with Mycobacterium tuberculosis are few and contradictory. The percentage of γ/δ T lymphocytes in the peripheral blood of tuberculin positive and tuberculin negative patients with Mycobacterium tuberculosis infection and healthy controls was compared. Methods - Thirty six patients infected with Mycobacterium tuberculosis and 11 healthy controls were studied. Lymphocytes were separated, cytocentrifuged onto glass microscope slides, and reacted with anti-γ/δ monoclonal antibody. The percentage of γ/δ positive cells was determined by microscopic counting of 300 lymphocytes. Results - No difference was found in the percentage of γ/δ T lymphocytes between patients and controls. However, when the patients were divided into two groups according to reactivity or non-reactivity in the Mantoux skin reaction a higher percentage of γ/δ T lymphocytes was found in the peripheral blood of patients with tuberculin anergy than in tuberculin positive patients or controls. Conclusions - Higher γ/δ T cell counts are found in tuberculin negative patients with tuberculosis than in tuberculin positive patients or tuberculin positive controls. The high γ/δ T cell counts in tuberculin anergic patients may reflect a shift in the immune response in a Th2 direction characterised by increased antibody production and decreased cell mediated responses.

Original languageEnglish
Pages (from-to)375-377
Number of pages3
JournalThorax
Volume52
Issue number4
Publication statusPublished - 1997

Fingerprint

Mycobacterium Infections
Mycobacterium tuberculosis
Tuberculin
T-Lymphocytes
Cell Count
Lymphocytes
Infection Control
Antibody Formation
Glass
Anti-Idiotypic Antibodies
Tuberculosis
Monoclonal Antibodies
Skin

Keywords

  • γ/δ T lymphocytes
  • Mycobacterium tuberculosis
  • Tuberculin anergy

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

Cite this

γ/δ T lymphocytes in Mycobacterium tuberculosis infection. / Balikó, Zoltán; Szereday, L.; Szekeres-Barthó, J.

In: Thorax, Vol. 52, No. 4, 1997, p. 375-377.

Research output: Contribution to journalArticle

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abstract = "Background - Data on the percentage of γ/δ T lymphocytes in the peripheral blood of patients infected with Mycobacterium tuberculosis are few and contradictory. The percentage of γ/δ T lymphocytes in the peripheral blood of tuberculin positive and tuberculin negative patients with Mycobacterium tuberculosis infection and healthy controls was compared. Methods - Thirty six patients infected with Mycobacterium tuberculosis and 11 healthy controls were studied. Lymphocytes were separated, cytocentrifuged onto glass microscope slides, and reacted with anti-γ/δ monoclonal antibody. The percentage of γ/δ positive cells was determined by microscopic counting of 300 lymphocytes. Results - No difference was found in the percentage of γ/δ T lymphocytes between patients and controls. However, when the patients were divided into two groups according to reactivity or non-reactivity in the Mantoux skin reaction a higher percentage of γ/δ T lymphocytes was found in the peripheral blood of patients with tuberculin anergy than in tuberculin positive patients or controls. Conclusions - Higher γ/δ T cell counts are found in tuberculin negative patients with tuberculosis than in tuberculin positive patients or tuberculin positive controls. The high γ/δ T cell counts in tuberculin anergic patients may reflect a shift in the immune response in a Th2 direction characterised by increased antibody production and decreased cell mediated responses.",
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T1 - γ/δ T lymphocytes in Mycobacterium tuberculosis infection

AU - Balikó, Zoltán

AU - Szereday, L.

AU - Szekeres-Barthó, J.

PY - 1997

Y1 - 1997

N2 - Background - Data on the percentage of γ/δ T lymphocytes in the peripheral blood of patients infected with Mycobacterium tuberculosis are few and contradictory. The percentage of γ/δ T lymphocytes in the peripheral blood of tuberculin positive and tuberculin negative patients with Mycobacterium tuberculosis infection and healthy controls was compared. Methods - Thirty six patients infected with Mycobacterium tuberculosis and 11 healthy controls were studied. Lymphocytes were separated, cytocentrifuged onto glass microscope slides, and reacted with anti-γ/δ monoclonal antibody. The percentage of γ/δ positive cells was determined by microscopic counting of 300 lymphocytes. Results - No difference was found in the percentage of γ/δ T lymphocytes between patients and controls. However, when the patients were divided into two groups according to reactivity or non-reactivity in the Mantoux skin reaction a higher percentage of γ/δ T lymphocytes was found in the peripheral blood of patients with tuberculin anergy than in tuberculin positive patients or controls. Conclusions - Higher γ/δ T cell counts are found in tuberculin negative patients with tuberculosis than in tuberculin positive patients or tuberculin positive controls. The high γ/δ T cell counts in tuberculin anergic patients may reflect a shift in the immune response in a Th2 direction characterised by increased antibody production and decreased cell mediated responses.

AB - Background - Data on the percentage of γ/δ T lymphocytes in the peripheral blood of patients infected with Mycobacterium tuberculosis are few and contradictory. The percentage of γ/δ T lymphocytes in the peripheral blood of tuberculin positive and tuberculin negative patients with Mycobacterium tuberculosis infection and healthy controls was compared. Methods - Thirty six patients infected with Mycobacterium tuberculosis and 11 healthy controls were studied. Lymphocytes were separated, cytocentrifuged onto glass microscope slides, and reacted with anti-γ/δ monoclonal antibody. The percentage of γ/δ positive cells was determined by microscopic counting of 300 lymphocytes. Results - No difference was found in the percentage of γ/δ T lymphocytes between patients and controls. However, when the patients were divided into two groups according to reactivity or non-reactivity in the Mantoux skin reaction a higher percentage of γ/δ T lymphocytes was found in the peripheral blood of patients with tuberculin anergy than in tuberculin positive patients or controls. Conclusions - Higher γ/δ T cell counts are found in tuberculin negative patients with tuberculosis than in tuberculin positive patients or tuberculin positive controls. The high γ/δ T cell counts in tuberculin anergic patients may reflect a shift in the immune response in a Th2 direction characterised by increased antibody production and decreased cell mediated responses.

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