β 2‐Microglobulin‐containing IgG Complexes in Sera and Synovial Fluids of Rheumatoid Arthritis and Systemic Lupus Erythematosus Patients

A. FALUS, K. MERÉTEY, G. GLIKMANN, S. ‐E SVEHAG, F. FÁBIÁN, U. BÖHM, S. BOZSÓKY

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Abstract

The occurrence and composition of complexed β2‐microglobulin (β2m) In sera and synovial fluids of rheumatoid arthritis and systemic lupus erythematosus patients and control persons was investigated. β2m‐containing complexes were detected In immune complex (IC)‐enriched fractions isolated by precipitation with 3% polyethylene glycol 6000, In the macromolecular peaks after Sephadex G‐200 gel filtration, and in IC desorbed from solid‐phase Clq. β2m complexes were demonstrated also after precipitation of redissolved PEG‐insoluble material by anti‐human β2m serum or isolation of the complexes by use of Sephadex‐anti‐β2m. IgG was co‐isolated with with β2m on Sephadex‐anti‐β2m, and free β2m inhibited the binding of IgG to Sephadex anti‐β2m, Indicating that IgG was present in the complexed β2m. Analysis by SDS–polyacrylamide gradient electrophoresis under reducing conditions indicated that the purified β2m complexes contained IgG and β2m.

Original languageEnglish
Pages (from-to)25-34
Number of pages10
JournalScandinavian Journal of Immunology
Volume13
Issue number1
DOIs
Publication statusPublished - Jan 1981

ASJC Scopus subject areas

  • Immunology

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