β-Lactam susceptibility patterns and investigation of cephalosporin hydrolysing β-lactamases of Gram-negative extraintestinal clinical isolates

Zsuzsanna Gál, Dóra Szabó, Péter Kovács, Ferenc Hernádi, Béla Tóth-Martinez, Ferenc Rozgonyi

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5 Citations (Scopus)

Abstract

Of more than 3500 isolates of enterobacteriaceae, 48-69% were resistant to aminopenicillins and 11-45% to amoxycillin + clavulanic acid. Resistance to second and third generation cephalosporins was present in 11-17 and 3-8% of Escherichia coli, 47-56 and 15-52% of Klebsiella-Enterobacter, 36-57 and 16-27% of Proteus, Providencia and Morganella isolates. Pseudomonas aeruginosa strains varied in their resistance to antipseudomonal β-lactams. Isoelectric points, inhibitor profiles and substrate profiles of β-lactamases extracted from representatives of the resistant strains indicated that the resistance was mainly due to the hyperproduction of chromosomally encoded AmpC β-lactamases. This was confirmed by plasmid profile and PCR investigations. Extended-spectrum β-lactamase and metallo-penicillinase producing strains were not found. One Pseudomonas maltophilia strain produced an oxacillinase. (C) 2000 Elsevier Science B.V. and International Society of Chemotherapy.

Original languageEnglish
Pages (from-to)395-400
Number of pages6
JournalInternational Journal of Antimicrobial Agents
Volume16
Issue number4
DOIs
Publication statusPublished - Dec 23 2000

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Keywords

  • AmpC β-lactamase
  • Cephalosporins
  • Characterisation of β-lactamases
  • Gram-negative bacteria
  • β-Lactam resistance

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases
  • Pharmacology (medical)

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