β-Glucosidase production of the diferent Aspergillus strains

A. Brumbauer, K. Réczey

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

β-Glucosidase has an important role in cellulose degradation by cleaving the cellobiose to glucose units. Supplementation of Trichoderma cellulase with exogenous β-glucosidase is needed to prevent the inhibition effect of cellobiose on exoglucanases and endoglucanases. Production of β-glucosidase by Aspergillus niger and Aspergillus phoenicis has been investigated under different fermentations. Both strains were appropriate for enzyme production at high level under the applied conditions. Cultivation of A. phoenicis on Mandels' medium containing a complex nitrogen source has resulted in a higher β-glucosidase activity than on Vogel's medium. In an air-lift fermenter A. phoenicis grew in the shape of beads with 1.41 IU g-1 cell-associated enzyme activity. The fungal pellets can be used as in situ immobilized enzyme preparation. A. niger produced extracellular β-glucosidase at the level of 2.1 IU ml-1 in stirred-tank fermenter with a yield of 210 IU g-1 glucose and productivity of 21.8 IU l-1 h-1.

Original languageEnglish
Pages (from-to)361-370
Number of pages10
JournalAccreditation and Quality Assurance
Volume4
Issue number12
Publication statusPublished - 1999

Fingerprint

Glucosidases
Niger
Aspergillus
glucose
enzymes
enzyme activity
fermentation
Fermenters
Cellobiose
productivity
cellulose
pellets
beads
Cellulase
Glucose
degradation
nitrogen
Enzymes
preparation
air

Keywords

  • β-Glucosidase
  • A. niger
  • A. phoenicis
  • Fermentation

ASJC Scopus subject areas

  • Analytical Chemistry
  • Instrumentation

Cite this

β-Glucosidase production of the diferent Aspergillus strains. / Brumbauer, A.; Réczey, K.

In: Accreditation and Quality Assurance, Vol. 4, No. 12, 1999, p. 361-370.

Research output: Contribution to journalArticle

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