β-amyloid[1-40]-induced early hyperpolarization in M26-1F cells, an immortalized rat striatal cell line

G. Laskay, M. Zarandi, K. Jost, B. Penke, E. Balint, I. Ocsovszki, M. Tarcsa, S. Varszegi, K. Gulya

Research output: Contribution to journalArticle

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Abstract

The short-term (20-minute) action of β[1-40]-amyloid on the resting transmembrane potential was investigated by means of flow-cytofluorimetric studies in M26-1F cells, an immortalized rat striatal cell line, using the potential-sensitive fluorescent probe bis-oxonol. The distribution of the individual cell-associated probe fluorescence was found to be shifted to lower levels in cells treated with β-amyloid[1-40] for 20 minutes as compared with that of their untreated counterparts. A change in the same direction was caused by valinomycin, a hyperpolarizing ionophore, whereas gramicidin, a depolarizing ionophore, induced a shift to higher fluorescence intensities. These findings, together with the reported behaviour of this particular fluorescent probe at different transmembrane potential levels, indicate that β-amyloid[1-40] is capable of inducing early hyperpolarization in M26-1F cells. This is one of the earliest cell physiological effect of β- amyloid peptides that has been reported so far. Moreover, our findings indicate an ionophore-like action of amyloid peptides.

Original languageEnglish
Pages (from-to)431-436
Number of pages6
JournalNeurobiology
Volume7
Issue number4
Publication statusPublished - 1999

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Corpus Striatum
Amyloid
Ionophores
Cell Line
Membrane Potentials
Fluorescent Dyes
Fluorescence
Valinomycin
Gramicidin
Peptides

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

β-amyloid[1-40]-induced early hyperpolarization in M26-1F cells, an immortalized rat striatal cell line. / Laskay, G.; Zarandi, M.; Jost, K.; Penke, B.; Balint, E.; Ocsovszki, I.; Tarcsa, M.; Varszegi, S.; Gulya, K.

In: Neurobiology, Vol. 7, No. 4, 1999, p. 431-436.

Research output: Contribution to journalArticle

Laskay, G, Zarandi, M, Jost, K, Penke, B, Balint, E, Ocsovszki, I, Tarcsa, M, Varszegi, S & Gulya, K 1999, 'β-amyloid[1-40]-induced early hyperpolarization in M26-1F cells, an immortalized rat striatal cell line', Neurobiology, vol. 7, no. 4, pp. 431-436.
Laskay, G. ; Zarandi, M. ; Jost, K. ; Penke, B. ; Balint, E. ; Ocsovszki, I. ; Tarcsa, M. ; Varszegi, S. ; Gulya, K. / β-amyloid[1-40]-induced early hyperpolarization in M26-1F cells, an immortalized rat striatal cell line. In: Neurobiology. 1999 ; Vol. 7, No. 4. pp. 431-436.
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AU - Zarandi, M.

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AU - Penke, B.

AU - Balint, E.

AU - Ocsovszki, I.

AU - Tarcsa, M.

AU - Varszegi, S.

AU - Gulya, K.

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