β-Amyloid-induced increase in the resting intracellular calcium concentration gives support to tell Alzheimer lymphocytes from control ones

András Palotás, J. Kálmán, Miklós Palotás, A. Juhász, Z. Janka, B. Penke

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Senile plaques containing β-amyloid peptide (βAP) comprise the major neuropathological lesions in Alzheimer's disease (AD). In line with ongoing studies investigating alterations of various biochemical processes of cells of peripheral tissues, the authors demonstrate differences in resting intracellular free calcium levels of lymphocytes harvested from sporadic Alzheimer patients and from age-matched controls. Resting intracellular calcium concentration was measured in Fura-2AM-loaded human lymphocytes by dual wavelength spectrofluorimetry. Resting calcium level appeared to be higher in Alzheimer cells when compared to control lymphocytes. After incubating cells in 10-7M of β-amyloid, the resting calcium concentration of the control cells elevated, while that of Alzheimer lymphocytes did not differ considerably.

Original languageEnglish
Pages (from-to)203-205
Number of pages3
JournalBrain Research Bulletin
Volume58
Issue number2
DOIs
Publication statusPublished - 2002

Fingerprint

Amyloid
Lymphocytes
Calcium
Biochemical Phenomena
Amyloid Plaques
Alzheimer Disease
Peptides

Keywords

  • β-Amyloid
  • Alzheimer's disease
  • Fluorescence
  • Lymphocyte
  • Resting calcium

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

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abstract = "Senile plaques containing β-amyloid peptide (βAP) comprise the major neuropathological lesions in Alzheimer's disease (AD). In line with ongoing studies investigating alterations of various biochemical processes of cells of peripheral tissues, the authors demonstrate differences in resting intracellular free calcium levels of lymphocytes harvested from sporadic Alzheimer patients and from age-matched controls. Resting intracellular calcium concentration was measured in Fura-2AM-loaded human lymphocytes by dual wavelength spectrofluorimetry. Resting calcium level appeared to be higher in Alzheimer cells when compared to control lymphocytes. After incubating cells in 10-7M of β-amyloid, the resting calcium concentration of the control cells elevated, while that of Alzheimer lymphocytes did not differ considerably.",
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T1 - β-Amyloid-induced increase in the resting intracellular calcium concentration gives support to tell Alzheimer lymphocytes from control ones

AU - Palotás, András

AU - Kálmán, J.

AU - Palotás, Miklós

AU - Juhász, A.

AU - Janka, Z.

AU - Penke, B.

PY - 2002

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AB - Senile plaques containing β-amyloid peptide (βAP) comprise the major neuropathological lesions in Alzheimer's disease (AD). In line with ongoing studies investigating alterations of various biochemical processes of cells of peripheral tissues, the authors demonstrate differences in resting intracellular free calcium levels of lymphocytes harvested from sporadic Alzheimer patients and from age-matched controls. Resting intracellular calcium concentration was measured in Fura-2AM-loaded human lymphocytes by dual wavelength spectrofluorimetry. Resting calcium level appeared to be higher in Alzheimer cells when compared to control lymphocytes. After incubating cells in 10-7M of β-amyloid, the resting calcium concentration of the control cells elevated, while that of Alzheimer lymphocytes did not differ considerably.

KW - β-Amyloid

KW - Alzheimer's disease

KW - Fluorescence

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