β-Aminobutyric acid-induced resistance against downy mildew in grapevine acts through the potentiation of callose formation and jasmonic acid signaling

Mollah Md Hamiduzzaman, G. Jakab, Laurent Barnavon, Jean Marc Neuhaus, Brigitte Mauch-Mani

Research output: Contribution to journalArticle

167 Citations (Scopus)

Abstract

β-Aminobutyric acid (BABA) was used to induce resistance in grapevine (Vitis vinifera) against downy mildew (Plasmopara viticola). This led to a strong reduction of mycelial growth and sporulation in the susceptible cv. Chasselas. Comparing different inducers, the best protection was achieved with BABA followed by jasmonic acid (JA), whereas benzo (1,2,3)-thiadiazole-7- carbothionic acid-S-methyl ester (a salicylic acid [SA] analog) and abscisic acid (ABA) treatment did not increase the resistance significantly. Marker genes for the SA and JA pathways showed potentiated expression patterns in BABA-treated plants following infection. The callose synthesis inhibitor 2-deoxy-D-glucose partially suppressed BABA- and JA-induced resistance against P. viticola in Chasselas. Application of the phenylalanine ammonia lyase inhibitor 2-aminoindan-2-phosphonic acid and the lipoxygenase (LOX) inhibitor 5, 8, 11, 14-eicosatetraynoic acid (ETYA) also led to a reduction of BABA-induced resistance (BABA-IR), suggesting that callose deposition as well as defense mechanisms depending on phenylpropanoids and the JA pathways all contribute to BABA-IR. The similar phenotype of BABA- and JA-induced resistance, the potentiated expression pattern of JA-regulated genes (LOX-9 and PR-4) following BABA treatment, and the suppression of BABA-IR with ETYA suggest an involvement of the JA pathway in BABA-IR of grapevine leading to a primed deposition of callose and lignin around the infection sites.

Original languageEnglish
Pages (from-to)819-829
Number of pages11
JournalMolecular Plant-Microbe Interactions
Volume18
Issue number8
DOIs
Publication statusPublished - Aug 2005

Fingerprint

Aminobutyrates
callose
downy mildew
induced resistance
jasmonic acid
acids
Plasmopara viticola
Salicylic Acid
lipoxygenase
salicylic acid
5,8,11,14-Eicosatetraynoic Acid
Genes
Phenylalanine Ammonia-Lyase
phosphorous acid
Lipoxygenase Inhibitors
Abscisic Acid
Acids
Lipoxygenase
Lignin
Deoxyglucose

Keywords

  • Oomycete

ASJC Scopus subject areas

  • Microbiology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biotechnology

Cite this

β-Aminobutyric acid-induced resistance against downy mildew in grapevine acts through the potentiation of callose formation and jasmonic acid signaling. / Hamiduzzaman, Mollah Md; Jakab, G.; Barnavon, Laurent; Neuhaus, Jean Marc; Mauch-Mani, Brigitte.

In: Molecular Plant-Microbe Interactions, Vol. 18, No. 8, 08.2005, p. 819-829.

Research output: Contribution to journalArticle

Hamiduzzaman, Mollah Md ; Jakab, G. ; Barnavon, Laurent ; Neuhaus, Jean Marc ; Mauch-Mani, Brigitte. / β-Aminobutyric acid-induced resistance against downy mildew in grapevine acts through the potentiation of callose formation and jasmonic acid signaling. In: Molecular Plant-Microbe Interactions. 2005 ; Vol. 18, No. 8. pp. 819-829.
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